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Nucleic Acid Extraction from Agrobacterium Strains
Time of Update: 2020-11-11
The successful use ofAgrobacterium as a tool for the genetic engineering of plant cells often requires the manipulation and analysis of nucleic acids present in recombinantAgrobacterium strains.
Here we present dependable methods for the isolation of genomic (total)DNA , mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA.
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Extraction and Analysis of Soluble Carbohydrates
Time of Update: 2020-11-11
During extraction and prior to analysis, steps should be taken to avoid change in form or quantity of sugars by endogenous active enzyme conversion or by contaminating microbial growth.
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White wax tree
Time of Update: 2020-11-11
A commonly known species of plant in the white wax tree genus of the wood rhinoceros.
This genus is about 70 species, mainly distributed in the temper belt of the northern hemisphere, a very small number of southwards extend to the tropics.
white wax tree is an important economic tree species in China.
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Laser Capture Microdissection of Nematode Feeding Cells
Time of Update: 2020-11-11
Feeding cell formation involves complex physiological and morphological changes to normal root cells and is accompanied by dramatic changes in plant gene expression.
Feeding cells comprise a small fraction of the total root cell population, and identification of plant gene expression changes specific to these cells is difficult.
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Measurement of Transcription Rates in Arabidopsis Chloroplasts
Time of Update: 2020-11-11
Here, we describe a protocol for run-on transcription in chloroplasts isolated from Arabidopsis leaves and present data on the transcriptional activity of several plastid genes in detached leaves of different Arabidopsis ecotypes.
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Lovewood
Time of Update: 2020-11-11
Bamboo peach Chloe wood belongs to evergreen shrubs, a medicinal plant.
Plant height 0.5 to 3 meters, multi-branched, with milky.
Single-leaf or 3 to 5 impliers, long oval-shaped needle-shaped.
A ovoid-shaped or oval shaped, raw and purple-black when ripe.
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Micropropagation of Gerbera (Gerbera jamesonii Bolus)
Time of Update: 2020-11-11
Rapid multiplication of elite cultivars ofGerbera , with improved agronomic traits, has been achieved by using both direct and indirect tissue culture methods.
Indirect shoot induction succeeded from callus differentiation has been achieved on MS medium containing 2 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L indole-3-acetic acid, and 2 mg/L BA.
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Introduction to Plant Virology
Time of Update: 2020-11-11
The requirement to work with viruses at the subcellular level drove technology to develop new tools for their study.
Because plant viruses are simple in structure and easy to purify, they are now ideal candidates for molecular studies.
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Transcriptomics on Small Samples
Time of Update: 2020-11-11
Among the challenges arising when extracting RNA from individual plant cells are: the isolation of pure cell populations; the small yield of material when isolating specific cell types, and ensuring an accurate representation of the transcriptome from each cell type after amplification of RNA.
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Paenoia albiflora
Time of Update: 2020-11-11
Flower single born at the top of the stem, flower terrier long, color white, yellow purple, pink, red and other colors, rarely light green, flower shape changes, there are single petals, heavy petals.
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Tomato (Lycopersicum esculentum)
Time of Update: 2020-11-11
We have used this protocol to generate transgenic lines from several tomato cultivars expressing various genes of interest and selectable markers.
We also provide protocols for molecular characterization of transgenic lines and batch processing tomato fruit.
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Protocols for Growing Plant Symbioses; Rhizobia
Time of Update: 2020-11-11
The high protein content of legume plants is achieved via the establishment of a root symbiosis with rhizobia that allows the reduction of atmospheric nitrogen.
The use of this plant as model in an increasing number of laboratories has resulted in the development of numerous protocols to study the establishment of the symbiosis.
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Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions
Time of Update: 2020-11-11
The yeast one-hybrid system is widely recognized as a valuable and straightforward technique to study interactions between transcription factors andDNA .
coli -yeast shuttle vector designed for production of hybrid proteins consisting of a library protein and thetrans -activating domain (AD) from the yeast GAL4 transcription factor.
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Target Preparation for DNA Microarray Hybridization
Time of Update: 2020-11-11
Before a nucleic acid (target) can be used for hybridization to the probes of a microarray, it needs to be extracted from the tissue and labeled.
During a hybridization process, labeled target molecules with sequences complementary to the probes are captured quantitatively.
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Plant Metabolomics by GC-MS and Differential Analysis
Time of Update: 2020-11-11
Metabolic fingerprinting, unlike metabolite profiling, does not aim at separating or identifying all the metabolites present in the sample, but rather generates a fingerprint that characterizes a specific metabolic state of the plant system under investigation.
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Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants
Time of Update: 2020-11-11
Plastid transformation technology offers attractive features in plant genetic engineering.
We demonstrated that this technology is a remarkable tool for the production of isoprenoids in plants through metabolic engineering.
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RNA Analysis: Size and 3 End Group Determination
Time of Update: 2020-11-11
Other reagents that denature but do not degrade RNA include formamide, which destroys base pairing, and glyoxal (ethanedial).
The second approach, which is based on the method of McMaster and Carmichael ( 3 ), uses glyoxal to denature the sample prior to loading it onto a nondenaturing gel.
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DNA Extraction from Arborescent Monocots and How to Deal with Other Challenging Hosts
Time of Update: 2020-11-11
Detection success depends largely upon the host species and sampling procedures and, to a lesser extent, on the protocol used for DNA extraction.
The protocol requires minimal processing of excised tissues and yields phytoplasma DNA preparations in suitable quantity for reliable detection by nested PCR assays.
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Sunflower Propagation
Time of Update: 2020-11-11
Several protocols have been described for the micropropagation, direct plant regeneration by organogenesis being acceptable for this plant species.
This chapter describes a protocol for direct shoot regeneration from cotyledons developed and established in our laboratory, as well as the improvement regenerated shoot quality.
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Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance
Time of Update: 2020-11-11
Herein, we describe the methods for the experimental measurements for flux analysis, i.e., determination of the biomass composition (lipid, protein, soluble sugar, and starch) as well as detailed procedures of acid hydrolysis of protein and starch samples and NMR sample preparation, using soybean embryo culture as the model plant system.
