Nucleic Acid Extraction from Agrobacterium Strains
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Last Update: 2020-11-11
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Source: Internet
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Author: User
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Agrobacterium
is routinely used as a tool for moving genetic constructs into plant cells. The successful use of
Agrobacterium
as a tool for the genetic engineering of plant cells often requires the manipulation and analysis of nucleic acids present in recombinant
Agrobacterium
strains. Here we present dependable methods for the isolation of genomic (total)
DNA
, mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA. In addition, we provide a simple method for the electronic transfer of shuttle plasmids from
Agrobacterium
to
E. coli
for use when their low copy number in
Agrobacterium
impedes plasmid isolation from that strain.
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