-
Cell-Free Transcription of SV40
Time of Update: 2021-02-01
The development of soluble cell-free systems that accurately transcribe genes has provided a means to investigate the mechanisms of eukaryotic transcription.
Our laboratory has been investigating the molecular mechanisms by which the genes of SV40 are transcriptionally regulated.
-
Measuring In Vivo Signaling Kinetics in a Mitogen-Activated Kinase Pathway Using Dynamic Input Stimulation
Time of Update: 2021-02-01
We can measure a property we termed pathway bandwidth to putin vivo bounds on the kinetics of the mitogen-activated protein kinase (MAPk) signaling cascade inSaccharomyces cerevisiae that responds to hyperosmotic stress [the High Osmolarity Glycerol (HOG) pathway].
-
Genome-Wide Approaches to Studying Yeast Chromatin Modifications
Time of Update: 2021-01-31
Techniques combining chromatin immunoprecipitation (ChIP) with microarrays (ChIP-chip) and second-generation sequencing (ChIP-Seq) have led to great advances in our understanding of how chromatin modifications contribute to gene regulation.
D. (2003) Genome-wide mapping of protein-DNA interactions by chromatin immunoprecipitation and DNA microarray hybridization.
-
Determination of Aflatoxins and Zearalenone in Different Culture Media
Time of Update: 2021-01-31
Some molds produce desirable changes in food, but most are merely esthetically undesirable.
These toxin substances, mycotoxins, are secondary metabolites produced by different fungi, especiallyAspergillus, Penicillium, Fusarium , and, to a lesser degree,Alternaria ( 1 ).
-
The Hairpin Ribozyme: Discovery and Development for Gene Therapy
Time of Update: 2021-01-31
The minimum catalytic center of (-)s TRSV was identified, biochemically characterized and named the hairpin ribozyme ( 1 ,2 ).
The minimum sequence for catalytic activity was found to be a 50 nucleotide (nt) ribozyme and a 14 nt substrate.
-
Simultaneous Direct Identification of Genital Microorganisms in Voided Urine Using Multiplex PCR-Based Reverse Line Blot Assays
Time of Update: 2021-01-31
Our aim was to develop and evaluate sensitive methods that would allow simultaneous direct identification of multiple potential pathogens in clinical specimens for diagnosis and epidemiological studies, using a multiplexPCR -based reverse line blot assay.
-
Lyssaviruses: Special Emphasis on Rabies Virus
Time of Update: 2021-01-31
Lyssavirus infection, better known as rabies, in animals or in humans, is characterized by a clinical picture of viral encephalitis.
The diagnosis of lyssavirus infection in animals, which may be responsible for human infections are of utmost importance: if positive, for starting or follow-up of postexposure rabies treatments, or if negative, for the nonprescription or interruption of the treatments.
-
The FERMEX Method for Metabolite-Enriched Fungal Extracts
Time of Update: 2021-01-31
The program was based on the observation that solid fermentations of fungi generally exhibited more complex metabolite profiles than when the same strains were grown on liquid medium.
To produce interference-free fermentations and improve organic product recovery, fungi colonized homogeneous media-saturated vermiculite thus promoting cellular and metabolic differentiation.
-
A Birds Eye View of the Bacterial Landscape
Time of Update: 2021-01-31
Bacteria have evolved four main types of bacterial cell surfaces to accomplish these functions: those of the typical gram-negative and gram-positive bacteria, and those of theActinobacteria andMollicutes .
-
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Time of Update: 2021-01-31
Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process.
Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion.
-
Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy
Time of Update: 2021-01-31
Dendritic cells (DC) are a family of bone-marrow-derived professional antigen presenting cells (APC) with sparse, but wide, tissue distribution ( 1 ).
-
Urinary Ethylenethiourea as a Biomarker of Exposure to Ethylenebisdithiocarbamates
Time of Update: 2021-01-31
A method for the determination or urinary ethylenethiourea (ETU), a major metabolite of ethylenebisdithiocarbamates (EBDTCs), is described.
A protocol for biological monitoring through determination of urinary ETU is provided.
In workers postexposure samples, median level of urinary ETU was 8.8 (from less than 0.5 to 126.3) �g/g creatinine.
-
Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction
Time of Update: 2021-01-31
Conditional expression–specialized transduction essentiality test (CESTET) is a genetic tool used to determine essentiality of individual genes inMycobacterium smegmatis .
Furthermore, effects of loss of essential gene function can then be studied by growing the transductants in medium depleted of acetamide.
-
Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori
Time of Update: 2021-01-31
Genetic manipulation ofHelicobacter pylori facilitates characterization and functional analysis of individualH.
pylori genes.
This chapter discusses the methods involved inH.
pylori chromosomalDNA isolation, mutagenesis of individual genes, and natural transformation.
-
Silver carp
Time of Update: 2021-01-31
It is a special fish from Heilongjiang, Yellow River, Yangtze River, Pearl River to Hainan Province and Northern Vietnam in eastern Asia.
In Hainan Province and northern Vietnam due to the warmer , its scale is larger, less , has formed there's the local species of large scale white slug.
-
Overview of Invasive Fungal Infections
Time of Update: 2021-01-31
As a direct response to this phenomenon, there has been a resolute effort over the past several decades to improve early and accurate diagnosis and provide reliable screening protocols thereby promoting the administration of appropriate antifungal therapy for fungal infections.
Early diagnosis and treatment with antifungal therapy are vital if a patient is to survive an IFI.
-
Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting
Time of Update: 2021-01-31
Saliva harbors a wide spectrum of proteins that may reflect the health/disease status in the human body.
Profiling of the proteins in saliva from a disease population can potentially yield valuable clinical parameters to be used for diagnosis and prognosis of the disease.
-
Use of Dodecahedron VLPs as an Alternative to the Whole Adenovirus
Time of Update: 2021-01-31
These form dodecahedral particles composed of 12 pentamers of penton base and 12 trimers of fiber protein.
These particles partly mimic the adenoviral cellular entry pathway but are devoid of genetic information making them an unusual tool for basic research or applications.
-
Isolation of Viruses from High Temperature Environments
Time of Update: 2021-01-31
The detection and isolation of viruses directly from high temperature (>80 C) acidic (pH6 cells/ml) and low free viral abundance (10 3 -10 6 particles/ml) compared to eutrophic freshwater lakes ( 1 ) or near- shore marine environments ( 2 ).
-
Construction of Retro viral Packaging Cell Lines
Time of Update: 2021-01-31
These sequences allow the recombinant structure to be transcribed (LTR promoter/enhancer) and the RNA to be processed (splicing and polyadenylation signals), packaged into a virion particle (packaging sequences), and replicated by the reverse transcriptase (tRNA binding site, R region, and polypurine track).
This can be simply achieved by using a replication-competent helper virus, leading to the production of a mixed population.
