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Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in Membrano and in Planta
Time of Update: 2020-11-15
The knowledge of the membrane lipid metabolism in photosynthetic cells is expected to benefit from the availability of inhibitors acting at the level of specific enzymes like MGD1 (E.C. 2.4.1.46) that catalyzes the synthesis of monogalactosyldiacylglycerol (MGDG) in chloroplasts.
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Tissue-Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination
Time of Update: 2020-11-15
Tissue printing provides a simple and rapid method to analyze localization of mRNA and protein at the tissue and cellular levels.
By the same principle, tissue-printing methods could also be valuable for elucidating the spatial characteristics of genes/proteins that control the inception, maintenance, and termination of seed dormancy.
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The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing
Time of Update: 2020-11-15
The combination of the use of a viroid-specific GFP-sensor, the grafting assays with plants silenced for the RDR6 and the analysis of siRNAs allows to address how to monitor viroid-specific RNA silencing and how to associate this regulatory pathway with symptom expression.
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Joint Actions of Baculoviruses and Other Control Agents
Time of Update: 2020-11-15
Several advantages are associated with the use of baculoviruses for pest control, including the host specificity and environmental compatibility attributes that offer major advantages over classical insecticides ( 2 ,3 ).
However, the success of baculoviruses as biological control agents of insect pests has been widely variable.
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Theobroma cacao
Time of Update: 2020-11-15
Leaves are born of each other, egg-shaped long round or inverted long round, slightly star-shaped hair on the veins, flower clusters on the trunk or main branch, diameter of about 1.8 cm; The part is sunken into a helmet-shaped, the upper spoon-shaped outward roll, the male flower silk base is combined to produce a barrel-shaped, degraded male 5, line-shaped, the development of male 1 to 3 pieces gathered into a group, and degraded males live in each other.
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Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice
Time of Update: 2020-11-15
Major advances in rice genomics during the last few years have made positional cloning in rice much more efficient.
This advanced strategy should be useful for the efficient identification of agronomic importantR genes from many resistant rice genotypes, including wild rice species.
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Inferring Biological Functions of Guanylyl Cyclases with Computational Methods
Time of Update: 2020-11-15
Here we describe how a computational based co-expression analysis can be used to link the function of a specific gene of interest to a defined cellular response.
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Side root
Time of Update: 2020-11-15
Usually the main root has a certain inhibitory effect on the growth of the side root, especially near the root end is more obvious.
If the root end of the main root is cut, the side root grows rapidly.
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Furovirus Isolation and RNA Extraction
Time of Update: 2020-11-15
The Furovirus ( fu ngus-transmittedro d-shaped) group, whose type member is soil-borne wheat mosaic virus (SBWMV) ( 1 ), is a very heterogeneous group infecting a wide variety of hosts.
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Analysis of the Synaptonemal Complex in Brassica Using TEM
Time of Update: 2020-11-15
Much of meiosis research is focussed onArabidopsis thaliana , largely due to the significant advantages it brings, having a small sequenced genome with comparatively little repetitiveDNA , the ease of forward and reverse genetics, and a short life cycle.
One solution is to use closely related species with larger genomes, in this case the Brassicas.
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Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
Time of Update: 2020-11-15
The shoot apical and floral meristems (SAM and FM, respectively) ofArabidopsis thaliana contain reservoirs of self-renewing stem cells that function as sources of progenitor cells for organ formation during development.
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Clonal Propagation of Cyclamen persicum Via Somatic Embryogenesis
Time of Update: 2020-11-15
Cyclamen ( Cyclamen persicum ) is an economically important ornamental pot plant with local use as cut flower as well.
This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.
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Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover
Time of Update: 2020-11-15
Insect bioassays showed that the transformed plants were highly toxic to bollworm larvae as well as obviously retarding development of aphid populations.
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Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques
Time of Update: 2020-11-15
As hybridization using conventional techniques has almost failed inTrifolium , embryo culture technique was used by breeders to obtain new combinations of interspecific hybrids.
Embryo culture has been effectively used in developing interspecific hybrids inTrifolium ambiguum ,T.
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Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions
Time of Update: 2020-11-15
By insertion into an individual xylem vessel at the root base, the multifunctional xylem probe allows the monitoring of the xylem pressure, the radial electrical gradients in the root (the so-called
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Accessing Integrated Brassica
Time of Update: 2020-11-15
The BASC system provides tools for integrated mining and browsing of genetic, genomic and phenotypic data.
We can use the tools within theBrassica BASC server to identify candidate genes for traits, conduct genome comparisons withArabidopsis , identify syntenic regions and view gene expression profiles.
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Leaf Hue Measurements: A High-Throughput Screening of Chlorophyll Content
Time of Update: 2020-11-15
Computer analysis of digital photographic images provides fast, high-throughput screening of leaf pigmentation.
Pixel-by-pixel conversion of red, green, blue (RGB) parameters to hue, saturation, value (HSV) showed that Hue values were proportional to total chlorophyll, offering an alternative to photometric analysis of leaf extracts.
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Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis
Time of Update: 2020-11-15
Transformation of theArabidopsis chloroplast genome was achieved via organogenesis but the efficiency was at least a 100-fold lower than in tobacco and had the drawback of polyploidy in the leaf tissue that resulted in sterile transgenic plants.
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Phorbol Esters
Time of Update: 2020-11-15
These are widely distributed in the plant species of the families Euphorbiaceae and Thymelaeaceae.
This chapter describes a method for determination of phorbol esters in physic nut ( Jatropha curcas ), which belongs to Euphorbiaceae family.
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Small-Molecule Dissection of Brassinosteroid Signaling
Time of Update: 2020-11-15
BR signaling is also regulated by the plant endocytic machinery because the increased endosomal localization of the BR receptor enhances the BR responses.
The compounds identified in this screen were used to dissect endomembrane trafficking of the BR receptor, BR INSENSITIVE1, a process that is essential for BR signal transduction.
