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Analysis of the Synaptonemal Complex in Brassica Using TEM

 Last Update: 2020-11-15
 Source: Internet
 Author: User

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silver

material cycle

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Much of meiosis research is focussed on
Arabidopsis thaliana
, largely due to the significant advantages it brings, having a small sequenced genome with comparatively little repetitive
DNA
, the ease of forward and reverse genetics, and a short life cycle. On the other hand, due the small genome size using
Arabidopsis
may be problematic for generating sufficient meiotic material for other types of analysis e.g., proteomics using prophase meiocytes and cytological analysis of the synaptonemal complex at the subcellular level. One solution is to use closely related species with larger genomes, in this case the Brassicas. This chapter contains methods for spreading of
Brassica oleracea
meiocytes for the analysis of the synaptonemal complex by silver staining and immunolocalization with gold-coupled antibodies using transmission electron microscopy.

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