-
Assay for Proteasome-Dependent Protein Degradation and Ubiquitinated Proteins
Time of Update: 2021-03-11
A transient expression system with tobacco leaves is a powerful method to evaluate E3 function and target degradation via UPS.
Here simple methods to assay proteasome-dependent protein degradation combined with a tobacco transient expression system and detection of accumulation of ubiquitinated proteins are presented.
-
A High-Efficiency Agrobacterium-Mediated Transformation System of Rice (Oryza sativa L.)
Time of Update: 2021-03-11
However, several elitejaponica and manyindica varieties cannot be efficiently transformed byAgrobacterium system.
In this system, the transformation efficiency of the calli is almost 100% in many varieties.
-
Birdsfoot trefoil
Time of Update: 2021-03-11
Hi warm and humid climate, barren, moisture-resistant, shade-resistant, can be planted between the forest fruit lines.
suitable for striping, with a line distance of 30-40cm, a depth of 1-2cm, and a planting volume of about 7-8kg per hectare.
-
Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat
Time of Update: 2021-03-11
In this chapter, we summarize the main features and challenges of wheat transformation and then describe detailed protocols for the production of transgenic wheat plants both by biolistic andAgrobacterium -mediatedDNA -delivery.
-
Rings
Time of Update: 2021-03-11
When in spring and summer, the climate is warm, the formation layer cells divide quickly, grow fast, the resulting wood cells are large, thin walls, more catheters, larger diameter, less wood fibers, loose wood, light and generous, called early or spring.
-
Flower pomegranate
Time of Update: 2021-03-11
Press bar, spring and autumn can be carried out, do not need to carve, buds before the germination with the root part of the branch pressed into the soil, after the summer root cut away from the mother plant, autumn can be seedling.
-
Apple coloring with a bag
Time of Update: 2021-03-10
First of all, the temperature should meet the apple tree growth and development, flowering results of the whole process, fruit ripening, a certain range of sustained low temperature, day and night temperature difference is greater conducive to the synthesis and accumulation of anthoside; apple bag technology is an effective measure to promote the coloring of red Fuji apples and improve the appearance of quality.
-
Bark
Time of Update: 2021-03-10
Broad concept refers to all tissues other than the formation layer of the stem (old trunk) and is the protective structure outside the trunk, i.e. the bark that can be peeled from the trunk during wood harvesting or processing and production.
-
Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens
Time of Update: 2021-03-10
The genetic transformation of many fungal species is well established and can be carried out by utilizing different transformation methods including electroporation, Agrobacterium, biolistics, or polyethylene glycol (PEG)-mediated transformation.
Here, we describe a PEG-based protocol developed for genetic transformation ofStagonospora nodorum , a fungal pathogen of wheat.
-
Trypsin Inhibitor
Time of Update: 2021-03-10
Among the many factors that have been implicated as having an adverse effect on the nutritional value of proteins is a class of proteins, known as protease inhibitors, that has the ability to inhibit
-
Plant Physiology
Time of Update: 2021-03-10
Plant life activities are very complex, can be artificially divided into water metabolism, mineral nutrition, photocodescing, respiratory action and innutation of and transport and other metabolic processes, and on the basis of the coordination of these processes, the normal growth and development process.
-
Corolla
Time of Update: 2021-03-10
In addition to the role of protecting females and males, the color, aroma, and honey secreted by the honey glands have the effect of attracting insects to pollinate.
-
Ivory red
Time of Update: 2021-03-10
Cut strong and full branches, 15-20 cm long, inserted into the sand bed, to maintain a humid environment, inserted 15-20 natural roots.
Dew cultivation, every winter trench fertilization, dry weather irrigation, to keep the soil moist: winter withering, timely renovation, conducive to the next spring new branches germination.
-
Umbrella (Umbelliferae)
Time of Update: 2021-03-10
a section of geminal leaf plants. Herbs, with secretion cavity, often contains aromatic oil, its ingredients to the main flavor of beans. Leaves are mutually existing, usually complex leaves, rare
-
Cataegus pinnatifida
Time of Update: 2021-03-10
Flower white, male 20 , flower column 3 to 5 , the base has soft hair.
China's Liaoning, Hebei, Henan, Shandong, Shanxi, Jiangsu and other places have been cultivated.
Variant mountain red, fruit larger, cultivated throughout North China.
-
Ask Jing (Equisetum arvense)
Time of Update: 2021-03-10
In the reproductive branch 1 a brush head-like spore leaf spike, so also known as pen head dish.
However, the leaves are still small brown scales, in the section formed a crucible-like, nutrient branches only for photochidying, do not produce spores, until autumn and winter withering.
-
Pollination
Time of Update: 2021-03-10
Self-pollination pollination method in which mature pollen from pollen sacs falls on the head of a pillar of the same flower.
-
Castor
Time of Update: 2021-03-10
Castor has long been planted sporadicly, harvesting castor seeds for medicine, oil extraction and handicrafts.
After entering the 20th century, due to the need for a large number of high-grade lubricants in modern industry, castor production developed rapidly.
-
Gene Expression Analysis of Shoot Apical Meristem Cell Types
Time of Update: 2021-03-10
Here we provide details of experimental methods involved in protoplasting of SAM cells, generating cell type-specific gene expression profiles, and analysis platforms for identifying and inferring gene networks.
-
Mapping Element Distributions in Plant Tissues Using Synchrotron X-ray Fluorescence Techniques
Time of Update: 2021-03-10
Synchrotron-based X-ray fluorescence (XRF) is allowing substantial advances in several disciplines of plant science by allowing the in situ examination of elements within plant tissues.
Here, we describe the general principles for designing and conducting experiments for the examination of elemental distributions in plant material using micro-XRF.
