A High-Efficiency Agrobacterium-Mediated Transformation System of Rice (Oryza sativa L.)
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Last Update: 2021-03-11
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Source: Internet
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Author: User
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Agrobacterium
-mediated transformation of rice has been routinely performed according to the protocol reported by Hiei et al. (Plant J. 6:271–282, 1994). However, several elite
japonica
and many
indica
varieties cannot be efficiently transformed by
Agrobacterium
system. Also a large number of transformants are required to generate T-
DNA
insertion and FOX libraries as well as gene-targeting studies. To overcome these challenges, we established a high-efficiency transformation system in rice by cocultivating rice calli with
Agrobacterium
on filter papers moistened with enriched liquid media instead of using solid media (Ozawa, Plant Sci. 176:522–527, 2009; Ozawa and Takaiwa, Plant Sci. 179:333–337, 2010). In this system, the transformation efficiency of the calli is almost 100% in many varieties.
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