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Gene Activation by T-DNA Tagging
Time of Update: 2021-03-13
T- DNA tagging has proven a powerful genetic approach for the isolation of novel plant genes.
Compared with other approaches to gene isolation (for a review,see ref.
Furthermore, T-DNA is an ideal gene tag, as it has the potential to insert preferentially into potentially transcribed regions of the genome ( 5 ).
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Key laboratory for fruit and vegetable preservation in Guangdong Province
Time of Update: 2021-03-13
related topics of the Guangdong Province fruit and vegetable preservation key laboratory is the Guangdong Provincial Science and Technology Department and Guangzhou Municipal Science and Technolo
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Seedless fruit
Time of Update: 2021-03-13
Bananas, for example, can develop directly into unseeded fruit without pollination and fertilization of female flowers in the lower part of the spiked flower sequence under natural conditions.
In addition to bananas, some varieties of citrus, persimmon, melon and grape can also form seedless fruits due to monogamical syringe under natural conditions.
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Determining Gene Flow in Transgenic Cotton
Time of Update: 2021-03-13
Gene flow is one of the major concerns associated with the release of transgenic plants into the environment.
Thus, it is important and also necessary to evaluate the extent of gene flow in the field for transgenic plants already released or being considered for a release.
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A Real-Time PCR Detection System for the Bois Noir and Flavescence Dore Phytoplasmas and Quantification of the Target DNA
Time of Update: 2021-03-13
The detection system described is reliable, specific, sensitive, and easily applicable to fast, high-throughput diagnosis of grapevine yellows phytoplasmas.
In addition to the detection system, an approach for the quantification of phytoplasmas in the sample is described.
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小孔律(law of small pore)
Time of Update: 2021-03-13
This is because there is an edge effect when the gas molecules spread outward from the hole surface.
The edge effect can be expressed, and there must be a certain distance between the holes so that the diffusers do not interfere with each other.
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Prunus mume
Time of Update: 2021-03-13
Flower leaves open, single or two out, barrel bell-shaped, petals are mostly white or pale red, fragrant.
Depending on the fruit color when harvesting, can be divided into white plum (green white), green plum (green), flower plum (with red) and other types.
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Pollen tube
Time of Update: 2021-03-13
Generally each pollen grain germinates to produce a pollen tube, with a poly-germination hole pollen grain can start to grow several pollen tubes at the same time, but in the end only one continues to grow.
The cytoste behind the cap area contains a variety of cytocytes, which are associated with the growth of pollen tubes.
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Camellia sinensis
Time of Update: 2021-03-13
Leaves contain coffee base, tea base, tannic acid, volatile oil, etc.
Its young leaves can be baked into tea, for the famous beverage, China's Lushan cloud tea, Fujian Wuyi rock tea and other well-known.
In addition to the beverage, and can be made of tea base, coffee base raw materials;
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Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis
Time of Update: 2021-03-13
In this chapter, we describe a protocol for in vivo [Ca 2+ ] cyt measurement in G-protein mutants expressing aequorin, a luminescent-based calcium biosensor, to extend our knowledge about G-protein mediated Ca 2+ signaling.
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Binding Studies
Time of Update: 2021-03-13
The primary stimulus initiating the sequence of events leading to such responses is the interaction of the hormone with its receptor protein.
In general, receptors may be either membrane-associated or soluble (or solubilized) and this chapter deals with one binding technique suitable for each class.
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Potato (Solanum tuberosum L.)
Time of Update: 2021-03-13
This chapter describes a rapid, efficient, and cost-effective system for the routine transformation of this crop plant at rates above 40% efficiency, calculated as the mean number of Southern blot-confirmed independent transgenics per number of internodal explants originally plated.
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Air pollution monitoring plants
Time of Update: 2021-03-13
When monitoring air pollution with monitoring plants, certain types of potted plants should be selected according to the specific types of pollutants emitted by the source of pollution, placed in areas requiring monitoring, and then observed and recorded symptoms and extent of their victimization.
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Transient Expression Assays in Tobacco Protoplasts
Time of Update: 2021-03-13
To discover gene functions, transient expression assays are a valuable tool because they can be done more rapidly and at a higher scale than generating stably transformed tissues.
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Gramineae
Time of Update: 2021-03-13
Leaves are mutually produced, two columns, consisting of leaves and leaves wrapped in straw.
In small spikes, they are then lined up in conical, total, spike or head-shaped flower sequences.
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Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems
Time of Update: 2021-03-13
Such plasticity in the composition of the thylakoid membrane protein complexes guarantees efficient function of the photosynthetic machinery.
In this chapter, we describe methods for separation of thylakoid membrane protein complexes at high resolution by two-dimensional gel electrophoretic systems.
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Helicobacteria
Time of Update: 2021-03-13
The 1st species is Vibrio, the cells are short, only once bent, arced, such as cholera bacteria, the 2 species is helicobacteria, the cells are 2 times or more Bending, spiral, tougher, such as small screw bacteria, type 3 is a helix, cells 2 or more bent into a spiral, cells soft, such as European regression heat dredging helix.
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Plant Single Cell Sampling
Time of Update: 2021-03-13
Plant single cell sampling and analysis allows the determination of solute concentrations in individual cells and tissues.
In this chapter, some selected single cell sampling and analysis methods are described in detail, and their advantages and possible pitfalls discussed.
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Experiment 14 Identification of volatile elements in plant tissue
Time of Update: 2021-03-13
pb(Ac) 2 H 2 S→PbS↓ 2HAc 2. Ba(OH) 2 CO 2 →BaCO 3 ↓ H 2 O 3. 2K 2 (HgI 4 ) 3NAaOH NH 3 → NH 2 Hg 2 O 4KI 3 From these reactions NaI 2H O can identify the presence of H 2 S, CO 2 , and NH 3 to demonstrate the volatile elements contained in plant tissue.
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Factory production of new varieties of plants
Time of Update: 2021-03-13
If we use pre-cultured plant cell protosomes for sophyte fusion, we can also quickly obtain a large number of hybrid cells.
addition, pollen cell protosumin culture and chromosomal double technology provides excellent technical means for rapid purification of specific plant line.