 Home > Biochemistry News > Plant Extracts News > Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems

Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems

 Last Update: 2021-03-13
 Source: Internet
 Author: User

Tags

gel electrophoresis machine

gel electrophoresis apparatus

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Photosynthetic machinery in the thylakoid membrane is prone to modifications depending on �environmental, developmental, and morphological parameters. Such plasticity in the composition of the thylakoid membrane protein complexes guarantees efficient function of the photosynthetic machinery. In this chapter, we describe methods for separation of thylakoid membrane protein complexes at high resolution by two-dimensional gel electrophoretic systems. Solubilization of the thylakoid membrane protein complexes either by dodecylmaltoside or digitonin is described first. Then, two partially overlapping �methods, blue native gel electrophoresis and high-resolution clear native gel electrophoresis, are demonstrated to separate the individual protein complexes. Finally, denaturing
SDS
-polyacrylamide gel electrophoresis is used to reveal the protein composition of each complex. Critical points in all protocols are addressed and representative examples of the composition of
Arabidopsis thaliana
thylakoid membrane protein complexes are shown.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Monobenzone CAS 103-16-2 White Crystal Powder USP/BP Standard

CAS No.: 103-16-2

Cosmetics Grade

$1-1.2/KG EXW

Carob Extract

CAS No.: 84961-45-5

pharmaceutical grade/food grade

$10-13/KG FOB

Premium ingredient Panax Ginseng Extract 80%

CAS No.: 50647-08-0

Food Grade

$25-30/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.