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Detection of siRNAs and miRNAs
Time of Update: 2020-11-18
Understanding these fundamental processes requires the sensitive and specific detection of small RNA species.
However, because of the small size of miRNAs and siRNAs, their detection is technically demanding.
Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.
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Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters
Time of Update: 2020-11-18
One of the major roles of JA is the versatile enhancement of the production of secondary metabolites that function as second messengers in plant defense responses.
Moreover, the advantages and disadvantages of the method are summarized and the relevant technical points are noted.
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Companion Protease Inhibitors to Protect Recombinant Proteins in Transgenic Plant Extracts
Time of Update: 2020-11-18
We describe a general approach for the use of recombinant protease inhibitors as stabilizing agents for clinically useful proteins extracted from transgenic plant tissues.
A procedure is first described to assess the overall (in)stability of heterologous proteins in transgenic plant crude protein extracts.
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Approaches to the Identification of ABAR as an Abscisic Acid Receptor
Time of Update: 2020-11-18
We implemented biochemical approaches, namely, the purification of ABA-binding proteins to identify a putative ABA receptor; this protein was further characterized by a combination of biochemical and reverse genetic approaches.
The identified ABA receptor, called ABAR, mediates the responses of plants to ABA in seed germination, postgerminative growth, and stomatal movement.
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In Silico Identification and Characterization of Effector Catalogs
Time of Update: 2020-11-18
Effectors are defined as those proteins that alter host cell structure and/or function by facilitating pathogen infection.
Here, we describe in detail how to identify and characterize effectors from a defined fungal proteome usingin silico techniques.
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IAA Analysis in Transgenic Plants
Time of Update: 2020-11-18
(5 ), which uses a combination of HPLC and fluorimetric detection of the derivatized IAA (see Fig. 1 ).
Extraction and quantitation of IAA using HPLC and fluorimetric detection of derivatized IAA.
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1H NMR-Based Metabolomics Methods for Chemical Genomics Experiments
Time of Update: 2020-11-18
Sortin 1 was applied to Arabidopsis seedlings to examine metabolic effects in a chemical genomics experiment and to demonstrate the utility of metabolomics in conjunction with other “omics” techniques.
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In Vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture
Time of Update: 2020-11-18
In vitro fertilization (IVF) systems using isolated male and female gametes have been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development, and early embryogenesis, since the female gametophytes of plants are deeply embedded within ovaries.
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In Vitro Prenylation Assay of Arabidopsis Proteins
Time of Update: 2020-11-18
InArabidopsis , hundreds of proteins involved in a great variety of biological processes are potential prenylation substrates that need to be verified, including heterotrimeric G proteins and most Rop and Rab small GTPases.
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Coriander.
Time of Update: 2020-11-18
Therefore, when the sun is short and the weather is cool in autumn (the south is late autumn and early winter), the yield of stems and leaves is high and the quality is good.
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Capsaicin Accumulation in Capsicum spp. Suspension Cultures
Time of Update: 2020-11-18
Fruits of chili peppers ( Capsicum spp.) specifically synthesize and accumulate a group of analogs known as capsaicinoids in the placenta tissues.
Several efforts have been focused to investigate the biosynthetic capacity of in vitro chili pepper cells and tissue cultures in order to determine the production feasibility of these compounds at the industrial level under controlled conditions.
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Plant and Crop Databases
Time of Update: 2020-11-18
The reader is directed to some of the major publicly available Internet-accessible relevant databases with summaries of the major focuses of those databases, and several examples are given to illustrate how to access plant genomics data.
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Immunolocalization of Meiotic Proteins in Arabidopsis thaliana: Method 2
Time of Update: 2020-11-18
Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements forArabidopsis cytology have provided a range of tools to investigate chromosome behavior during meiosis (Jones et al.
Chromosome Res 11:205–215, 2003).
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Gene Regulation During Cold Stress Acclimation in Plants
Time of Update: 2020-11-18
Diverse plant species tolerate cold stress to a varying degree, which depends on reprogramming gene expression to modify their physiology, metabolism, and growth.
Cold signal in plants is transmitted to activate CBF-dependent (C-repeat/drought-responsive element binding factor-dependent) and CBF-independent transcriptional pathway, of which CBF-dependent pathway activates CBF regulon.
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Ambrosiaspp
Time of Update: 2020-11-18
This genus is about 10 species, mainly produced in the Americas.
Both are native to North America, in recent years in China's northeast, north China, central China are seen to produce weeds, both are included in China's quarantine weeds, its pollen is the main allergenic source of pollen allergenic disease.
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Cloud solid
Time of Update: 2020-11-18
After plant watering, after germination, the use of thin fertilizer, to promote the growth of side branches.
Properly trim plastic, 4-May flowering season at any time to remove the withering flower sequence, to promote the germination of new branches.
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Brown Algae as a Model for Plant Organogenesis
Time of Update: 2020-11-18
The use of these and other emerging brown algal models will undoubtedly add to our knowledge on the mechanisms that regulate development in multicellular photosynthetic organisms.
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An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil
Time of Update: 2020-11-18
An accelerated soil bait test can be used to determine whether a field harbours virus-carryingSpongospora subterranea .
This test may be used to assess the risk of planting potato crops in a particular field.
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The Dicot Root as a Model System for Studying Organogenesis
Time of Update: 2020-11-18
While lateral organ formation at the shoot and root might seem quite different, recent genetic studies have highlighted numerous parallels between these processes.
In this chapter we describe the conceptual and technical advantages that support lateral root development as a good model system for studying organogenesis in plants.
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Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways
Time of Update: 2020-11-18
Functional complementation of yeast mutants, displaying selectable phenotypes, has been used very successfully in the past years to isolate many plant genes involved in signalling, stress response or metabolic pathways.
