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Many characterized fungal effector proteins are small secreted proteins. Effectors are defined as those proteins that alter host cell structure and/or function by facilitating pathogen infection. The identification of effectors by molecular and cell biology techniques is a difficult task. However, with the availability of whole-genome sequences, these proteins can now be predicted
in silico
. Here, we describe in detail how to identify and characterize effectors from a defined fungal proteome using
in silico
techniques.