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Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Time of Update: 2020-11-09
Phytopathogenic fungi, due to its particular cellular characteristics, can be considered as a recalcitrant biological material, which makes it difficult to obtain quality protein samples for proteomic analysis.
This chapter focuses on protein extraction for gel- and LC-based proteomics with specific protocols of our current research withBotrytis cinerea .
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Medicago truncatula Transformation Using Cotyledon Explants
Time of Update: 2020-11-09
By using cotyledons as explants forAgrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes.
Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo afterAgrobacterium -mediated transformation.
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The Potential Roles of microRNAs in Molecular Breeding
Time of Update: 2020-11-09
Moreover, artificial target mimics are applied for the reduction of specific miRNA activity.
Consequently, miRNA-based manipulations have emerged as promising new approaches for the improvement of crop plants.
Herein, we describe the current miRNA-based plant engineering approaches, and their advantages and challenges are also stated.
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Falling Peanuts (Arachis hypogaea)
Time of Update: 2020-11-09
The silkworm-shaped oval shape, 2 to 5 cm long, the surface has mesh, the seed between the micro-shrink, do not crack; Originally from Brazil and Peru in South America, has nearly 2000 years of cultivation history, in 1492 from South America into Europe, 15 to 16 centuries into China, china's four major oil crops.
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Targeted Biolistics for Improved Transformation of Impatiens balsamina
Time of Update: 2020-11-09
These tissues can be disrupted by the helium blast effect associated with conventional biolistic devices, so we have utilised modifications to the PDS 1000/He equipment originally developed for transformation of fragile insect tissues.
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Reverse Genetics in Rice Using Tos17
Time of Update: 2020-11-09
We report here two protocols for ascertaining the presence of aTos17 insertion in a gene of interest among R2/T2 seeds received fromTos17 mutant stock centers: The first protocol isPCR -based and allows the identification of azygous, heterozygous and homozygous plants among progenies segregating the insertion.
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Crested wheatgrass
Time of Update: 2020-11-09
Widely distributed in the cold and arid grasslands of the Asian part of the former Soviet Union, china's northeast, northwest and north China are wild.
Suitable for autumn or summer sowing, pre-planting need to fertilize the whole land, sowing capacity of 11-22 kg per hectare.
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Rapid System for Evaluating Bioproduction Capacity of Complex Pharmaceutical Proteins in Plants
Time of Update: 2020-11-09
We have optimized a simple transient expression system inNicotiana benthamiana enabling rapid assessment of transgene potential for plant-based bioproduction.
This system provides a rapid source of transgene product for assessing posttranslational modifications, purification strategies, and bioactivity as well as an effective system for optimizing construct elements.
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Identification and Characterization of Cyclic Nucleotide Phosphodiesterases
Time of Update: 2020-11-09
Cyclic nucleotide phosphodiesterases regulate cellular levels of small molecule second messengers that control important biological processes in all kingdoms of life. Identifying and characterizing t
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The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers
Time of Update: 2020-11-09
The cellular mechanisms that some plant species use to tolerate Al are still not understood today.
Plant cell culture has been used for the investigation of both: Al-toxicity and -tolerance.
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Mung bean.
Time of Update: 2020-11-09
Poor environmental conditions are often prone to the production of thick skin, poor water absorption, not easy to germinate hard, its hard rate is slightly higher than other edible beans.
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Chestnuts (Castanea mollissima)
Time of Update: 2020-11-09
The leaves are round or long round, with jagged jagged teeth on the edges, green on top, shiny on top, and gray-white hair on the bottom.
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Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen
Time of Update: 2020-11-09
While NCOs may represent a large fraction of meiotic recombination events in plants, as in many other higher eukaryotes, they have been poorly characterized due to the technical difficulty of detecting them.
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Heterologous Production of Fungal Effectors in Pichia pastoris
Time of Update: 2020-11-09
In this chapter a method for the heterologous production of fungal proteins in the yeastPichia pastoris is described.
pastoris transformation, production of the protein in a fermentor, and purification of the protein.
This method has successfully been used for the production of a number of fungal effector proteins.
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Proteomics of Field Samples in Radioactive Chernobyl Area
Time of Update: 2020-11-09
Two serious nuclear accidents during the past quarter of a century contaminated large agricultural areas with radioactivity.
The remediation and possible recovery of radio-contaminated areas for agricultural purposes require comprehensive characterization of plants grown in such places.
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In Silico Mining and PCR-Based Approaches to Transcription Factor Discovery in Non-model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers
Time of Update: 2020-11-09
Identification of WRKY genes in these non-model plants is equally important for understanding the evolutionary and function-adaptive processes of this transcription factor family.
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Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
Time of Update: 2020-11-09
Using OLIMP, we can harness the selective power of a specific wall hydrolase together with the speed and sensitivity of mass spectrometry to provide highly reproducible structural and compositional information about the wall molecule of interest.
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Trees.
Time of Update: 2020-11-09
Such as oil pine, hair poplar, water fir and so on.
Leaf to autumn and winter is dead called deciduous trees, such as ginkgo; According to the height of the trees can be divided into: height of more than 25 meters called large trees, height of 8 to 25 meters of trees, height of less than 8 meters of trees called small trees.
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Preparation of Phytoplasma Membrane Recombinant Proteins
Time of Update: 2020-11-09
The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol
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Quantification of the Antioxidant Activity in Salt-Stressed Tissues
Time of Update: 2020-11-09
Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods.
Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase.
