-
Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen
Time of Update: 2020-11-09
While NCOs may represent a large fraction of meiotic recombination events in plants, as in many other higher eukaryotes, they have been poorly characterized due to the technical difficulty of detecting them.
-
Chestnuts (Castanea mollissima)
Time of Update: 2020-11-09
The leaves are round or long round, with jagged jagged teeth on the edges, green on top, shiny on top, and gray-white hair on the bottom.
-
Trees.
Time of Update: 2020-11-09
Such as oil pine, hair poplar, water fir and so on.
Leaf to autumn and winter is dead called deciduous trees, such as ginkgo; According to the height of the trees can be divided into: height of more than 25 meters called large trees, height of 8 to 25 meters of trees, height of less than 8 meters of trees called small trees.
-
Preparation of Phytoplasma Membrane Recombinant Proteins
Time of Update: 2020-11-09
The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol
-
Quantification of the Antioxidant Activity in Salt-Stressed Tissues
Time of Update: 2020-11-09
Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods.
Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase.
-
Identification and Characterization of Cyclic Nucleotide Phosphodiesterases
Time of Update: 2020-11-09
Cyclic nucleotide phosphodiesterases regulate cellular levels of small molecule second messengers that control important biological processes in all kingdoms of life. Identifying and characterizing t
-
Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
Time of Update: 2020-11-09
Using OLIMP, we can harness the selective power of a specific wall hydrolase together with the speed and sensitivity of mass spectrometry to provide highly reproducible structural and compositional information about the wall molecule of interest.
-
The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers
Time of Update: 2020-11-09
The cellular mechanisms that some plant species use to tolerate Al are still not understood today.
Plant cell culture has been used for the investigation of both: Al-toxicity and -tolerance.
-
The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves
Time of Update: 2020-11-09
In this chapter we focus on its use in molecular plant pathology, and especially for the expression of plant resistance ( R ) and fungal avirulence ( Avr ) (effector) genes in leaves ofNicotiana benthamiana .
-
Soilless cultivation of commonly used substititures - rice shells
Time of Update: 2020-11-09
Carbonized rice shell weight 0.15g/cm 3 , total porosity 82.5%, large pore 57.5%, small pore 25.0%, water-to-air ratio of 1:0.43, pH 6.5.
After the carbonization of rice shell, pH is often more than 9.0, must be washed or adjusted with acid after use, in order to ensure the normal growth and development of crops.
-
Proteomic Techniques for PlantFungal Interactions
Time of Update: 2020-11-09
More and more pathogen genomes are being sequenced, so fungal proteomics is reaching its full potential and remains the chosen technology to unravel the molecular pathways of pathogenicity and resistance.
In this chapter, we suggest proteomic strategies that have proved successful on various plant-interacting fungal species.
-
Ganoderma lucidum.
Time of Update: 2020-11-09
The lid (in-cap) is semi-circular or kidney-shaped, the initial yellow after gradually turning red-brown, the appearance of paint-like luster, with ring-shaped ribs and radiant wrinkles, there are many small holes under the lid, white or hazel, for the hole tube mouth.
-
Challenges Facing Seed Banks and Agriculture in Relation to Seed Quality
Time of Update: 2020-11-09
Seeds form a convenient vehicle for storage of germplasm, both for agricultural purposes and conservation of wild species.
When required, seeds can be taken from storage and germinated, and plants can be propagated for the desired purpose, e.g., crop production or biome restoration.
-
Plant Proteomics: From Genome Sequencing to Proteome Databases and Repositories
Time of Update: 2020-11-09
Proteomic approaches are useful for the identification of functional proteins.
These have been enhanced not only by the development of proteomic techniques but also in concert with genome sequencing.
-
Transgenic Cotton: From Biotransformation Methods to Agricultural Application
Time of Update: 2020-11-09
Transgenic cotton is among the first transgenic plants commercially adopted around the world.
Agrobacterium -mediated genetic transformation method is the major method for obtaining transgenic cotton.
However, pollen tube pathway-mediated method is also used, particularly by scientists in China, to breed commercial transgenic cotton.
-
Transformation of Maize via Tissue Electroporation
Time of Update: 2020-11-09
Introduction ofDNA into plant protoplasts via electroporation is a well-known procedure. By giving electrical impulses of high field strength, the cell membrane is reversibly permeabilized, so that
-
Spring snow taro
Time of Update: 2020-11-09
Breeding: can be used sub-plant method, spring to summer for the appropriate period, the plant can grow young plants, sub-plants planted into new plants.
Spring to summer is the fertility period, water should be sufficient.
-
Horn daffodils
Time of Update: 2020-11-09
Low-temperature treatment of the ball, after the plant if the temperature of 25 degrees C, will seriously affect the effect of spring.
-
Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis
Time of Update: 2020-11-09
Although vast numbers of mutants have been identified in Arabidopsis, the continued use of forward-genetic screening approaches remains valuable for the isolation and study of previously overlooked mutants and novel mutations in sensitised backgrounds (i.e., suppressors or enhancers of previously known mutants).
-
Fungicide Resistance Assays for Fungal Plant Pathogens
Time of Update: 2020-11-09
Laboratory assays are used to determine loss of sensitivity, or resistance, to a fungicide and can explain fungicide failures and for developing successful fungicide recommendations in the field.
Laboratory assays for fungicide resistance are conducted by measuring reductions in growth or spore germination of fungi in the presence of fungicide, or by molecular procedures.
