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01 Microsatellite instability (MSI) is one of the most important detection targets in colorectal cancer, and it is of great clinical significance.
It is necessary to clarify its appropriate detection panel and clinicopathological characteristics
.
02 We have compiled a study of the East China population in Frontiers in Genetics [1], which completed the data analysis of MSI and clinicopathological characteristics of 2356 patients with colorectal cancer
.
MSI-H and BRAF mutations are more common in 03RCC (right colon cancer) (p=0.
000; p=0.
001)
.
04 The overall positive detection rate of MSI was only 6.
3%.
With reference to other studies reported previously, the overall positive rate was significantly lower.
This may be due to the insufficient sensitivity of the panel detection (6 single nucleotides, NR21, NR24, NR27, NR21, NR24, NR27, BAT25, BAT26, MONO27) or the bias of the enrolled patient population and other reasons
.
The relevant clinicopathological and molecular pathological characteristics of 2356 patients with colorectal cancer were collected and sorted out, including gender, age, TNM stage, tumor stage, gross classification, degree of differentiation, MSI status, KRAS gene mutation, and NRAS gene mutation.
, BRAF gene mutations and other data
.
Finally, compare whether there are significant differences in the above clinical case characteristics or molecular pathological characteristics in left colorectal cancer (LCRC) and right colorectal cancer (RCC)
.
The final data showed that in 2356 patients with colorectal cancer, RCC accounted for about 20%, and RCC was more common with younger patients (p=0.
000), the risk of metastasis was higher (p=0.
003), and the degree of differentiation was lower (p= 0.
000)
.
In addition, in all samples, the positive detection rate of KRAS mutation was 40.
15%, the positive detection rate of NRAS mutation was 3.
85%, the positive detection rate of BRAF mutation was 2.
3%, and the detection rate of MSI-H was 6.
31%
.
MSI-H and BRAF mutations are more common in RCC (p=0.
000; p=0.
001)
.
The research team compared the data findings of the study with previous research reports
.
In this study, RCC accounted for 18.
25%, slightly lower than the research data of other parts of China (24.
4%-50.
6%), Japan (26.
3%-29.
3%) and the United States (42%)
.
Nevertheless, the clinicopathological characteristics of RCC and LCRC are similar to previous data.
RCC is more common in young patients, female patients, metastatic patients, poorly differentiated adenocarcinoma, and is highly correlated with BRAF mutation positive and MSI-H
.
From the perspective of molecular pathological characteristics, the positive rates of KRAS and NRAS gene mutations are consistent with previous data reports, while the positive rates of MSI-H and BRAF genes are 6.
31% and 2.
29%, respectively, which are lower than previous reported data
.
It is worth noting that among the more than 2000 cases of colorectal cancer in the study, the detection rate of MSI-H in the East China population was only 6.
31%, which was lower than the incidence of MSI-H in the Chinese population in other studies (10%-20%)
.
The reason may be regional differences.
If it is a regional difference, more research data may be needed to explore
.
In addition, it is also possible that MSI-H was missed to a certain extent in this study
.
The site selection used in this study is 6 single-nucleotide sites (NR21, NR24, NR27, BAT25, BAT26, MONO27), which is not the 2B3D NCI panel (BAT-25, BAT-25, BAT-25, BAT-25, BAT-25, BAT25, BAT26, MONO27).
-26, D5S346, D2S123, D17S250) or 5 single nucleotide position Panel (Promega: BAT25, BAT26, NR21, NR24, MONO27)
.
Therefore, there may be a certain risk of missed detection, resulting in a low detection rate
.
We have summarized the data of all Chinese populations that have been published based on PCR-CE method, but different MSI detection Panel
.
In summary, the overall positive rate of 2B3D NCI panel is 13.
5%, Promega Panel 12.
9% (5 single nucleotides), and 6 single nucleotide Panels are only 7.
7%, basically repeating the above results
.
In summary, the study collected 2356 patients with colorectal cancer and found that LCRC and RCC have different clinical and molecular pathological characteristics in the East China population
.
In the study, the investigator also mentioned that in view of the differences between the results of the study and the data of other regions in Europe, America and China, it is necessary to clarify the clinical and molecular pathological characteristics of LCRC and RCC in colorectal cancer.
More research data
.
In addition, the choice of MSI detection panel is also very important.
The detection rate of MSI-H at 6 single nucleotide sites is low
.
References 1.
Song YL, Wang LL, Ran WW, et al.
Effect of Tumor Location on Clinicopathological and Molecular Markers in Colorectal Cancer in Eastern China Patients: An Analysis of 2,356 Cases.
Front Genet 2020;11:96.
2.
LI X, LIU QH.
Study on Microsatellite Instability in Colorectal Cancer Patients with Familial Predisposition.
J China Med Univ 2006;37:410-408.
3.
Jin P, Meng XM, Sheng JQ, et al.
Clinicopathological Features of Non-familial Colorectal Cancer with High- frequency Microsatellite Instability.
Chin Med Sci J 2010;25:228-232.
4.
Yang BL, Gu YF.
Microsatellite instability in sporadic colorectal cancer and its relationship with clinicopathological features.
World Chinese Journal of Digestology 2007;15:1160-1164.
5.
Jin P , Meng XM, Sheng JQ, et al.
Clinicopathological Features of Non-familial Colorectal Cancer with High-frequency Microsatellite Instability.
Chin Med Sci J 2010;25:228-232.
6.
Meng WJ, Wang L, Yu YY, et al.
Significance of microsatellite instability in sporadic stage II and III rectal cancer.
Chongqing Med J 2010;39:2420-2426.
7.
Peng JL, Tang T, Ye ZL, et al.
The relationship of microsatellite instability state with loss of mismatch repair proteins and clinical pathological characteristics in sporadic colorectal cancers.
Chin J Cancer Biother 2015;22:479-483.
8.
Zhou LY, Wan MZ, Liu YP, et al.
Assessment of Microsatallite Instability in Colorectal Carcinoma: A Comparison Between Immunohistochemistry and PCR Method.
Cancer Res Prev Treat 2015;42:1231-1234.
9.
Yan WY , Hu J, Xie L, et al.
Prediction of biological behavior and prognosis of colorectal cancer patients by tumor MSI/MMR in the Chinese population.
Onco Targets Ther 2016;9:7415-7424.
10.
Zheng JM, Huang BX, Nie X, et al.
The clinicopathological features and prognosis of tumor MSI in East Asian colorectal cancer patients using NCI panel.
Future Oncol 2018;14:1355-1364.
11.
Bai WQ, Ma JF, Liu YY, et al.
Screening of MSI detection loci and their heterogeneity in East Asian colorectal cancer patients.
Cancer Med 2019;8:2157-2166.
12.
Jiang N.
Relationship between microsatellite instability and clinicopathological features and prognosis in sporadic colorectal cancer.
Acta Universitatis Medicinalis Anhui 2019;54:139-142.
13.
Huang YQ, Yuan Y, Ge WT, et al.
Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients.
J Zhejiang Univ-SC B 2010;11:647-653.
14.
Li WQ, Li HN, Liu RQ, et al.
Comprehensive Analysis of the Relationship Between RAS and RAF Mutations and MSI Status of Colorectal Cancer in Northeastern China.
Cell Physiol Biochem 2018 ;50:1496-1509.
Wang Z, Tang XL, Wu XQ, et al.
Mismatch Repair status between primary colorectal tumor and metastatic tumor, a retrospective consistent study.
Biosci Rep 2019;39:BSR20190730.
It is necessary to clarify its appropriate detection panel and clinicopathological characteristics
.
02 We have compiled a study of the East China population in Frontiers in Genetics [1], which completed the data analysis of MSI and clinicopathological characteristics of 2356 patients with colorectal cancer
.
MSI-H and BRAF mutations are more common in 03RCC (right colon cancer) (p=0.
000; p=0.
001)
.
04 The overall positive detection rate of MSI was only 6.
3%.
With reference to other studies reported previously, the overall positive rate was significantly lower.
This may be due to the insufficient sensitivity of the panel detection (6 single nucleotides, NR21, NR24, NR27, NR21, NR24, NR27, BAT25, BAT26, MONO27) or the bias of the enrolled patient population and other reasons
.
The relevant clinicopathological and molecular pathological characteristics of 2356 patients with colorectal cancer were collected and sorted out, including gender, age, TNM stage, tumor stage, gross classification, degree of differentiation, MSI status, KRAS gene mutation, and NRAS gene mutation.
, BRAF gene mutations and other data
.
Finally, compare whether there are significant differences in the above clinical case characteristics or molecular pathological characteristics in left colorectal cancer (LCRC) and right colorectal cancer (RCC)
.
The final data showed that in 2356 patients with colorectal cancer, RCC accounted for about 20%, and RCC was more common with younger patients (p=0.
000), the risk of metastasis was higher (p=0.
003), and the degree of differentiation was lower (p= 0.
000)
.
In addition, in all samples, the positive detection rate of KRAS mutation was 40.
15%, the positive detection rate of NRAS mutation was 3.
85%, the positive detection rate of BRAF mutation was 2.
3%, and the detection rate of MSI-H was 6.
31%
.
MSI-H and BRAF mutations are more common in RCC (p=0.
000; p=0.
001)
.
The research team compared the data findings of the study with previous research reports
.
In this study, RCC accounted for 18.
25%, slightly lower than the research data of other parts of China (24.
4%-50.
6%), Japan (26.
3%-29.
3%) and the United States (42%)
.
Nevertheless, the clinicopathological characteristics of RCC and LCRC are similar to previous data.
RCC is more common in young patients, female patients, metastatic patients, poorly differentiated adenocarcinoma, and is highly correlated with BRAF mutation positive and MSI-H
.
From the perspective of molecular pathological characteristics, the positive rates of KRAS and NRAS gene mutations are consistent with previous data reports, while the positive rates of MSI-H and BRAF genes are 6.
31% and 2.
29%, respectively, which are lower than previous reported data
.
It is worth noting that among the more than 2000 cases of colorectal cancer in the study, the detection rate of MSI-H in the East China population was only 6.
31%, which was lower than the incidence of MSI-H in the Chinese population in other studies (10%-20%)
.
The reason may be regional differences.
If it is a regional difference, more research data may be needed to explore
.
In addition, it is also possible that MSI-H was missed to a certain extent in this study
.
The site selection used in this study is 6 single-nucleotide sites (NR21, NR24, NR27, BAT25, BAT26, MONO27), which is not the 2B3D NCI panel (BAT-25, BAT-25, BAT-25, BAT-25, BAT-25, BAT25, BAT26, MONO27).
-26, D5S346, D2S123, D17S250) or 5 single nucleotide position Panel (Promega: BAT25, BAT26, NR21, NR24, MONO27)
.
Therefore, there may be a certain risk of missed detection, resulting in a low detection rate
.
We have summarized the data of all Chinese populations that have been published based on PCR-CE method, but different MSI detection Panel
.
In summary, the overall positive rate of 2B3D NCI panel is 13.
5%, Promega Panel 12.
9% (5 single nucleotides), and 6 single nucleotide Panels are only 7.
7%, basically repeating the above results
.
In summary, the study collected 2356 patients with colorectal cancer and found that LCRC and RCC have different clinical and molecular pathological characteristics in the East China population
.
In the study, the investigator also mentioned that in view of the differences between the results of the study and the data of other regions in Europe, America and China, it is necessary to clarify the clinical and molecular pathological characteristics of LCRC and RCC in colorectal cancer.
More research data
.
In addition, the choice of MSI detection panel is also very important.
The detection rate of MSI-H at 6 single nucleotide sites is low
.
References 1.
Song YL, Wang LL, Ran WW, et al.
Effect of Tumor Location on Clinicopathological and Molecular Markers in Colorectal Cancer in Eastern China Patients: An Analysis of 2,356 Cases.
Front Genet 2020;11:96.
2.
LI X, LIU QH.
Study on Microsatellite Instability in Colorectal Cancer Patients with Familial Predisposition.
J China Med Univ 2006;37:410-408.
3.
Jin P, Meng XM, Sheng JQ, et al.
Clinicopathological Features of Non-familial Colorectal Cancer with High- frequency Microsatellite Instability.
Chin Med Sci J 2010;25:228-232.
4.
Yang BL, Gu YF.
Microsatellite instability in sporadic colorectal cancer and its relationship with clinicopathological features.
World Chinese Journal of Digestology 2007;15:1160-1164.
5.
Jin P , Meng XM, Sheng JQ, et al.
Clinicopathological Features of Non-familial Colorectal Cancer with High-frequency Microsatellite Instability.
Chin Med Sci J 2010;25:228-232.
6.
Meng WJ, Wang L, Yu YY, et al.
Significance of microsatellite instability in sporadic stage II and III rectal cancer.
Chongqing Med J 2010;39:2420-2426.
7.
Peng JL, Tang T, Ye ZL, et al.
The relationship of microsatellite instability state with loss of mismatch repair proteins and clinical pathological characteristics in sporadic colorectal cancers.
Chin J Cancer Biother 2015;22:479-483.
8.
Zhou LY, Wan MZ, Liu YP, et al.
Assessment of Microsatallite Instability in Colorectal Carcinoma: A Comparison Between Immunohistochemistry and PCR Method.
Cancer Res Prev Treat 2015;42:1231-1234.
9.
Yan WY , Hu J, Xie L, et al.
Prediction of biological behavior and prognosis of colorectal cancer patients by tumor MSI/MMR in the Chinese population.
Onco Targets Ther 2016;9:7415-7424.
10.
Zheng JM, Huang BX, Nie X, et al.
The clinicopathological features and prognosis of tumor MSI in East Asian colorectal cancer patients using NCI panel.
Future Oncol 2018;14:1355-1364.
11.
Bai WQ, Ma JF, Liu YY, et al.
Screening of MSI detection loci and their heterogeneity in East Asian colorectal cancer patients.
Cancer Med 2019;8:2157-2166.
12.
Jiang N.
Relationship between microsatellite instability and clinicopathological features and prognosis in sporadic colorectal cancer.
Acta Universitatis Medicinalis Anhui 2019;54:139-142.
13.
Huang YQ, Yuan Y, Ge WT, et al.
Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients.
J Zhejiang Univ-SC B 2010;11:647-653.
14.
Li WQ, Li HN, Liu RQ, et al.
Comprehensive Analysis of the Relationship Between RAS and RAF Mutations and MSI Status of Colorectal Cancer in Northeastern China.
Cell Physiol Biochem 2018 ;50:1496-1509.
Wang Z, Tang XL, Wu XQ, et al.
Mismatch Repair status between primary colorectal tumor and metastatic tumor, a retrospective consistent study.
Biosci Rep 2019;39:BSR20190730.
