The mechanism of Lactobacillus casei Zhang in alleviating kidney disease

Kidney diseases mainly include Acute Kidney Injury (AKI) and Chronic Kidney Disease (CKD). The main treatments for acute kidney injury include symptomatic supportive treatment and renal replacement therapy. The current mainstream symptomatic treatments often have insufficient therapeutic effects. , And easy to further develop into chronic kidney disease . Scientific research has shown that the structural imbalance of the intestinal flora is related to the damage of the intestinal mucosal barrier, inflammation of the kidney disease, oxidative stress and kidney damage . As an important microecological regulator, probiotics can well regulate the structure of human intestinal flora . A research team from Tongji Hospital of Huazhong University of Science and Technology has carried out scientific research on the improvement of Ketuo Biostar strain Lactobacillus casei Zhang (LC-Zhang), including acute kidney injury and chronic kidney disease, and explored its mechanism of action . The results found that: 1. LC-Zhang reduces kidney injury and fibrosis. 　　This experiment uses mice to conduct a randomized controlled experiment, and is divided into a control group and a treatment group (including LC-Zhang and Lactobacillus acidophilus), and ischemia-reperfusion Treatment to induce an acute kidney injury model . At the same time, the damaged mouse kidneys were observed and found that compared with the control group, the damage of the junction between the renal cortex and the medulla of the oral LC-Zhang treatment group was lighter . In addition, compared with the Lactobacillus acidophilus treatment group and the control group, the oral LC-Zhang treatment group can effectively reduce serum urea nitrogen (BUN) and creatinine levels, and there are fewer pathological damages such as necrosis and renal tubule expansion . Through immunofluorescence staining, it was found that the expression of specific fibrosis markers a-SMA and collagen I in the LC-Zhang treatment group was significantly reduced . In summary, it is shown that Lactobacillus casei Zhang can reduce acute kidney injury and fibrosis, and the effect is better than Lactobacillus acidophilus . 　　2. LC-Zhang improves the imbalance of intestinal flora 　　acute kidney injury induced by ischemia-reperfusion treatment can lead to imbalance of intestinal flora, which analyzed the diversity of ɑ . The experiment found that after LC-Zhang treatment, the diversity index of the intestinal flora of mice was significantly higher than that of the control group and the Lactobacillus acidophilus treatment group, and the diversity index between the control group and the Lactobacillus acidophilus treatment group was no Obvious difference . After LC-Zhang treatment, it was found that the abundance of short-chain fatty acid (SCFA)-producing bacteria (Alloprevotella and Prevotellaceae) in the mouse intestinal flora was significantly increased . This indicates that Lactobacillus casei Zhang can improve the imbalance of intestinal flora caused by kidney damage and increase the content of beneficial bacteria such as short-chain fatty acid (SCFA) production . 　　3. LC-Zhang improves inflammation and intestinal mucosal barrier damage 　　through immunofluorescence staining, intestinal damage and inflammation caused by kidney damage can be determined . First, the intestinal permeability was tested and found that LC-Zhang reduced the intestinal permeability; in addition, the level of inflammatory factors was tested and found that the tumor necrosis factor (TNF-a) level in the LC-Zhang treatment group was lower . This indicates that Lactobacillus casei Zhang can better improve intestinal inflammation and intestinal mucosal barrier damage caused by kidney damage .   　　LC-Zhang may improve kidney damage by inducing an increase in short-chain fatty acids (SCFA): While LC-Zhang improves the intestinal flora, it also increases the content of short-chain fatty acids (SCFA) and other beneficial bacteria, thereby increasing propionate, The content of short-chain fatty acids (SCFA) such as butyrate reduces the infiltration of macrophages and neutrophils in the kidney tissue, thereby maintaining a low level of renal interstitial fibrosis and intestinal inflammation .   　　4. LC-Zhang reduces renal function decline in chronic kidney disease   　　Regarding LC-Zhang's improvement in chronic kidney disease, the research team of Tongji Hospital immediately recruited 62 patients with chronic kidney disease in stages 3-5, and randomly divided them into a probiotic group (each given 1 billion CFU/day) and a placebo group , And detect renal function indicators such as creatinine, urea nitrogen, parathyroid hormone (PTH) and glomerular filtration rate (eGFR) before and after the test . Three months later, it was found that LC-Zhang intervention effectively reduced the serum cystatin C level, inhibited the significant increase in serum PTH, and also inhibited the increase in urine albumin-creatinine ratio (UACR) . One year later, it was found that LC-Zhang intervention effectively inhibited the increase of creatinine level . At the same time, LC-Zhang intervention also delayed the decline of eGFR . LC-Zhang can not only regulate the intestinal flora, but also delay the decrease of nicotinamide content caused by drugs, thereby maintaining the stability of nicotinamide content . In summary, it shows that Lactobacillus casei Zhang slowed down the development of chronic kidney disease and slowed down the decline of its renal function .   　　 Summary 　　Lactobacillus casei Zhang can not only regulate the imbalance of intestinal flora caused by kidney disease, increase the content of beneficial bacteria, thereby increasing the content of short-chain fatty acids; it can also reduce the intestinal inflammation and kidney fibrosis caused by acute kidney injury . In the test for chronic kidney disease, it was found that it can inhibit the decline of kidney function and maintain the content of nicotinamide . It is reported that the results of this research have been successfully published in "Cell Metabolism" (IF=27. 287) . 　　The star strain-Lactobacillus casei Zhang 　　was isolated from natural fermented mare milk in the Xilin Gol prairie of Inner Mongolia in 2002. It is the first lactic acid bacteria in China to complete the whole genome sequencing . Through scientific research, it has been found that Lactobacillus casei Zhang can regulate the intestinal flora, improve immunity, prevent upper respiratory tract infections in middle-aged and elderly people, and regulate the rejuvenation of intestinal flora . As of August 2021, 16 related patents have been applied for Lactobacillus casei Zhang and 176 papers have been published   Disclaimer: This article only represents the author's personal views and has nothing to do with China Probiotics. com . The originality and the text and content stated in the article have not been verified by this site. 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　　Kidney diseases mainly include Acute Kidney Injury (AKI) and Chronic Kidney Disease (CKD).
The main treatments for acute kidney injury include symptomatic supportive treatment and renal replacement therapy.
The current mainstream symptomatic treatments often have insufficient therapeutic effects.
, And easy to further develop into chronic kidney disease
.
Scientific research has shown that the structural imbalance of the intestinal flora is related to the damage of the intestinal mucosal barrier, inflammation of the kidney disease, oxidative stress and kidney damage
.
As an important microecological regulator, probiotics can well regulate the structure of human intestinal flora
.


　　A research team from Tongji Hospital of Huazhong University of Science and Technology has carried out scientific research on the improvement of Ketuo Biostar strain Lactobacillus casei Zhang (LC-Zhang), including acute kidney injury and chronic kidney disease, and explored its mechanism of action
.
The results found that:

　　1.
LC-Zhang reduces kidney injury and fibrosis.


　　This experiment uses mice to conduct a randomized controlled experiment, and is divided into a control group and a treatment group (including LC-Zhang and Lactobacillus acidophilus), and ischemia-reperfusion Treatment to induce an acute kidney injury model
.
At the same time, the damaged mouse kidneys were observed and found that compared with the control group, the damage of the junction between the renal cortex and the medulla of the oral LC-Zhang treatment group was lighter
.
In addition, compared with the Lactobacillus acidophilus treatment group and the control group, the oral LC-Zhang treatment group can effectively reduce serum urea nitrogen (BUN) and creatinine levels, and there are fewer pathological damages such as necrosis and renal tubule expansion
.
Through immunofluorescence staining, it was found that the expression of specific fibrosis markers a-SMA and collagen I in the LC-Zhang treatment group was significantly reduced
.
In summary, it is shown that Lactobacillus casei Zhang can reduce acute kidney injury and fibrosis, and the effect is better than Lactobacillus acidophilus
.



　　2.
LC-Zhang improves the imbalance of intestinal flora

　　acute kidney injury induced by ischemia-reperfusion treatment can lead to imbalance of intestinal flora, which analyzed the diversity of ɑ
.
The experiment found that after LC-Zhang treatment, the diversity index of the intestinal flora of mice was significantly higher than that of the control group and the Lactobacillus acidophilus treatment group, and the diversity index between the control group and the Lactobacillus acidophilus treatment group was no Obvious difference
.
After LC-Zhang treatment, it was found that the abundance of short-chain fatty acid (SCFA)-producing bacteria (Alloprevotella and Prevotellaceae) in the mouse intestinal flora was significantly increased
.
This indicates that Lactobacillus casei Zhang can improve the imbalance of intestinal flora caused by kidney damage and increase the content of beneficial bacteria such as short-chain fatty acid (SCFA) production
.



　　3.
LC-Zhang improves inflammation and intestinal mucosal barrier damage

　　through immunofluorescence staining, intestinal damage and inflammation caused by kidney damage can be determined
.
First, the intestinal permeability was tested and found that LC-Zhang reduced the intestinal permeability; in addition, the level of inflammatory factors was tested and found that the tumor necrosis factor (TNF-a) level in the LC-Zhang treatment group was lower
.
This indicates that Lactobacillus casei Zhang can better improve intestinal inflammation and intestinal mucosal barrier damage caused by kidney damage
.

Summary

　　Lactobacillus casei Zhang can not only regulate the imbalance of intestinal flora caused by kidney disease, increase the content of beneficial bacteria, thereby increasing the content of short-chain fatty acids; it can also reduce the intestinal inflammation and kidney fibrosis caused by acute kidney injury
.
In the test for chronic kidney disease, it was found that it can inhibit the decline of kidney function and maintain the content of nicotinamide
.
It is reported that the results of this research have been successfully published in "Cell Metabolism" (IF=27.
287)
.


　　The star strain-Lactobacillus casei Zhang

　　was isolated from natural fermented mare milk in the Xilin Gol prairie of Inner Mongolia in 2002.
It is the first lactic acid bacteria in China to complete the whole genome sequencing
.
Through scientific research, it has been found that Lactobacillus casei Zhang can regulate the intestinal flora, improve immunity, prevent upper respiratory tract infections in middle-aged and elderly people, and regulate the rejuvenation of intestinal flora
.
As of August 2021, 16 related patents have been applied for Lactobacillus casei Zhang and 176 papers have been published

---

　　Kidney diseases mainly include Acute Kidney Injury (AKI) and Chronic Kidney Disease (CKD).
The main treatments for acute kidney injury include symptomatic supportive treatment and renal replacement therapy.
The current mainstream symptomatic treatments often have insufficient therapeutic effects.
, And easy to further develop into chronic kidney disease
.
Scientific research has shown that the structural imbalance of the intestinal flora is related to the damage of the intestinal mucosal barrier, inflammation of the kidney disease, oxidative stress and kidney damage
.
As an important microecological regulator, probiotics can well regulate the structure of human intestinal flora
.


　　A research team from Tongji Hospital of Huazhong University of Science and Technology has carried out scientific research on the improvement of Ketuo Biostar strain Lactobacillus casei Zhang (LC-Zhang), including acute kidney injury and chronic kidney disease, and explored its mechanism of action
.
The results found that:

　　1.
LC-Zhang reduces kidney injury and fibrosis.


　　This experiment uses mice to conduct a randomized controlled experiment, and is divided into a control group and a treatment group (including LC-Zhang and Lactobacillus acidophilus), and ischemia-reperfusion Treatment to induce an acute kidney injury model
.
At the same time, the damaged mouse kidneys were observed and found that compared with the control group, the damage of the junction between the renal cortex and the medulla of the oral LC-Zhang treatment group was lighter
.
In addition, compared with the Lactobacillus acidophilus treatment group and the control group, the oral LC-Zhang treatment group can effectively reduce serum urea nitrogen (BUN) and creatinine levels, and there are fewer pathological damages such as necrosis and renal tubule expansion
.
Through immunofluorescence staining, it was found that the expression of specific fibrosis markers a-SMA and collagen I in the LC-Zhang treatment group was significantly reduced
.
In summary, it is shown that Lactobacillus casei Zhang can reduce acute kidney injury and fibrosis, and the effect is better than Lactobacillus acidophilus
.



　　2.
LC-Zhang improves the imbalance of intestinal flora

　　acute kidney injury induced by ischemia-reperfusion treatment can lead to imbalance of intestinal flora, which analyzed the diversity of ɑ
.
The experiment found that after LC-Zhang treatment, the diversity index of the intestinal flora of mice was significantly higher than that of the control group and the Lactobacillus acidophilus treatment group, and the diversity index between the control group and the Lactobacillus acidophilus treatment group was no Obvious difference
.
After LC-Zhang treatment, it was found that the abundance of short-chain fatty acid (SCFA)-producing bacteria (Alloprevotella and Prevotellaceae) in the mouse intestinal flora was significantly increased
.
This indicates that Lactobacillus casei Zhang can improve the imbalance of intestinal flora caused by kidney damage and increase the content of beneficial bacteria such as short-chain fatty acid (SCFA) production
.



　　3.
LC-Zhang improves inflammation and intestinal mucosal barrier damage

　　through immunofluorescence staining, intestinal damage and inflammation caused by kidney damage can be determined
.
First, the intestinal permeability was tested and found that LC-Zhang reduced the intestinal permeability; in addition, the level of inflammatory factors was tested and found that the tumor necrosis factor (TNF-a) level in the LC-Zhang treatment group was lower
.
This indicates that Lactobacillus casei Zhang can better improve intestinal inflammation and intestinal mucosal barrier damage caused by kidney damage
.

Summary

　　Lactobacillus casei Zhang can not only regulate the imbalance of intestinal flora caused by kidney disease, increase the content of beneficial bacteria, thereby increasing the content of short-chain fatty acids; it can also reduce the intestinal inflammation and kidney fibrosis caused by acute kidney injury
.
In the test for chronic kidney disease, it was found that it can inhibit the decline of kidney function and maintain the content of nicotinamide
.
It is reported that the results of this research have been successfully published in "Cell Metabolism" (IF=27.
287)
.


　　The star strain-Lactobacillus casei Zhang

　　was isolated from natural fermented mare milk in the Xilin Gol prairie of Inner Mongolia in 2002.
It is the first lactic acid bacteria in China to complete the whole genome sequencing
.
Through scientific research, it has been found that Lactobacillus casei Zhang can regulate the intestinal flora, improve immunity, prevent upper respiratory tract infections in middle-aged and elderly people, and regulate the rejuvenation of intestinal flora
.
As of August 2021, 16 related patents have been applied for Lactobacillus casei Zhang and 176 papers have been published

---

　　Kidney diseases mainly include Acute Kidney Injury (AKI) and Chronic Kidney Disease (CKD).
The main treatments for acute kidney injury include symptomatic supportive treatment and renal replacement therapy.
The current mainstream symptomatic treatments often have insufficient therapeutic effects.
, And easy to further develop into chronic kidney disease
.
Scientific research has shown that the structural imbalance of the intestinal flora is related to the damage of the intestinal mucosal barrier, inflammation of the kidney disease, oxidative stress and kidney damage
.
As an important microecological regulator, probiotics can well regulate the structure of human intestinal flora
.


　　A research team from Tongji Hospital of Huazhong University of Science and Technology has carried out scientific research on the improvement of Ketuo Biostar strain Lactobacillus casei Zhang (LC-Zhang), including acute kidney injury and chronic kidney disease, and explored its mechanism of action
.
The results found that:

　　1.
LC-Zhang reduces kidney injury and fibrosis.


　　This experiment uses mice to conduct a randomized controlled experiment, and is divided into a control group and a treatment group (including LC-Zhang and Lactobacillus acidophilus), and ischemia-reperfusion Treatment to induce an acute kidney injury model
.
At the same time, the damaged mouse kidneys were observed and found that compared with the control group, the damage of the junction between the renal cortex and the medulla of the oral LC-Zhang treatment group was lighter
.
In addition, compared with the Lactobacillus acidophilus treatment group and the control group, the oral LC-Zhang treatment group can effectively reduce serum urea nitrogen (BUN) and creatinine levels, and there are fewer pathological damages such as necrosis and renal tubule expansion
.
Through immunofluorescence staining, it was found that the expression of specific fibrosis markers a-SMA and collagen I in the LC-Zhang treatment group was significantly reduced
.
In summary, it is shown that Lactobacillus casei Zhang can reduce acute kidney injury and fibrosis, and the effect is better than Lactobacillus acidophilus
.

　　2.
LC-Zhang improves the imbalance of intestinal flora

　　acute kidney injury induced by ischemia-reperfusion treatment can lead to imbalance of intestinal flora, which analyzed the diversity of ɑ
.
The experiment found that after LC-Zhang treatment, the diversity index of the intestinal flora of mice was significantly higher than that of the control group and the Lactobacillus acidophilus treatment group, and the diversity index between the control group and the Lactobacillus acidophilus treatment group was no Obvious difference
.
After LC-Zhang treatment, it was found that the abundance of short-chain fatty acid (SCFA)-producing bacteria (Alloprevotella and Prevotellaceae) in the mouse intestinal flora was significantly increased
.
This indicates that Lactobacillus casei Zhang can improve the imbalance of intestinal flora caused by kidney damage and increase the content of beneficial bacteria such as short-chain fatty acid (SCFA) production
.

　　3.
LC-Zhang improves inflammation and intestinal mucosal barrier damage

　　through immunofluorescence staining, intestinal damage and inflammation caused by kidney damage can be determined
.
First, the intestinal permeability was tested and found that LC-Zhang reduced the intestinal permeability; in addition, the level of inflammatory factors was tested and found that the tumor necrosis factor (TNF-a) level in the LC-Zhang treatment group was lower
.
This indicates that Lactobacillus casei Zhang can better improve intestinal inflammation and intestinal mucosal barrier damage caused by kidney damage
.


　　LC-Zhang may improve kidney damage by inducing an increase in short-chain fatty acids (SCFA): While LC-Zhang improves the intestinal flora, it also increases the content of short-chain fatty acids (SCFA) and other beneficial bacteria, thereby increasing propionate, The content of short-chain fatty acids (SCFA) such as butyrate reduces the infiltration of macrophages and neutrophils in the kidney tissue, thereby maintaining a low level of renal interstitial fibrosis and intestinal inflammation
.

　　4.
LC-Zhang reduces renal function decline in chronic kidney disease 　　Regarding LC-Zhang's improvement in chronic kidney disease, the research team of Tongji Hospital immediately recruited 62 patients with chronic kidney disease in stages 3-5, and randomly divided them into a probiotic group (each given 1 billion CFU/day) and a placebo group , And detect renal function indicators such as creatinine, urea nitrogen, parathyroid hormone (PTH) and glomerular filtration rate (eGFR) before and after the test
.
Three months later, it was found that LC-Zhang intervention effectively reduced the serum cystatin C level, inhibited the significant increase in serum PTH, and also inhibited the increase in urine albumin-creatinine ratio (UACR)
.
One year later, it was found that LC-Zhang intervention effectively inhibited the increase of creatinine level
.
At the same time, LC-Zhang intervention also delayed the decline of eGFR
.
LC-Zhang can not only regulate the intestinal flora, but also delay the decrease of nicotinamide content caused by drugs, thereby maintaining the stability of nicotinamide content
.
In summary, it shows that Lactobacillus casei Zhang slowed down the development of chronic kidney disease and slowed down the decline of its renal function
.

　　
Summary

　　Lactobacillus casei Zhang can not only regulate the imbalance of intestinal flora caused by kidney disease, increase the content of beneficial bacteria, thereby increasing the content of short-chain fatty acids; it can also reduce the intestinal inflammation and kidney fibrosis caused by acute kidney injury
.
In the test for chronic kidney disease, it was found that it can inhibit the decline of kidney function and maintain the content of nicotinamide
.
It is reported that the results of this research have been successfully published in "Cell Metabolism" (IF=27.
287)
.


　　The star strain-Lactobacillus casei Zhang

　　was isolated from natural fermented mare milk in the Xilin Gol prairie of Inner Mongolia in 2002.
It is the first lactic acid bacteria in China to complete the whole genome sequencing
.
Through scientific research, it has been found that Lactobacillus casei Zhang can regulate the intestinal flora, improve immunity, prevent upper respiratory tract infections in middle-aged and elderly people, and regulate the rejuvenation of intestinal flora
.
As of August 2021, 16 related patents have been applied for Lactobacillus casei Zhang and 176 papers have been published

---