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The advantages and disadvantages of PCR technology for microbial detection

 Last Update: 2022-03-18
 Source: Internet
 Author: User

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(1) Advantages of PCR technology for microbial detection

The traditional method of detecting microorganisms usually uses the plate culture method, and the bacterial colony concentration is judged by the colony count

(2) Disadvantages of PCR technology for microbial detection

PCR technology also has some shortcomings, so it has not yet become a routine inspection method

PCR is used for microbial detection, and the biggest problem is false positives caused by nucleic acid contamination

If the template solution used for PCR amplification contains a certain component that inhibits the PCR reaction, it may cause the amplification to fail and result in false negatives

The PCR method detects microorganisms, and the results are expressed as "presence" or "absence", which can only be qualitatively discriminated and cannot be quantitatively analyzed

Related links: Principles and operating procedures of PCR technology to detect microorganisms

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