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Translation: Yue RuiRiediger et al.
published an online research paper entitled Analysis of a Photosynthetic Cyanobacterium Rich in Internal Membrane Systems via Gradient Profiling by Sequencing (Grad-seq) in The Plant Cell, which explored the RNA in a photosynthetic cyanobacteria -Protein complex.
Background: Cyanobacteria are a special kind of bacteria that can carry out photosynthesis similar to plants.
Similar to terrestrial plants and eukaryotic algae, they only need light, inorganic nutrients, and relatively stable environmental conditions to survive, but these factors often change and are unpredictable.
Cyanobacteria have developed corresponding mechanisms to deal with these changes.
One of these mechanisms is also a common mechanism in all life forms, which is gene regulation at the transcriptional level.
However, since it takes a certain amount of time to take effect, only this is the only mechanism.
One mechanism is usually not enough.
Therefore, post-transcriptional regulation is the same or more important mechanism.
Proteins that bind and interact with regulatory small RNAs and other RNAs are important in the process of post-transcriptional regulation, but they have been rarely studied in cyanobacteria.
Scientific question: What are the RNA-binding proteins involved in important post-transcriptional regulation processes in cyanobacteria that have not been clearly studied in other bacteria? The post-transcriptional regulation of cyanobacteria and other non-photosynthetic bacteria may be very different.
Research results: We conducted a global analysis of the main and stable RNA-protein complex in a photosynthetic cyanobacterium.
Cyanobacteria have complex intracellular membrane systems, and these membrane systems tend to bind protein complexes.
These characteristics make cyanobacteria and plants photosynthesize cells, especially chloroplasts, similar, but distinguish them from other bacteria.
We explored Synechocystis sp.
PCC 6803, the most widely studied model cyanobacterium.
We analyzed the sedimentation reaction of 65% in 3681 different proteins and 62.
2% in 4251 different transcription units by sucrose density gradient.
We calculated a support vector machine score for predicting RNA-binding proteins, and mainly analyzed proteins with unknown function that co-sedimented with RNAs.
Assuming that these proteins are more conservative in phylogenetic evolution, we searched for possible homologs in cyanobacteria, Arabidopsis, Escherichia coli, and Salmonella enterica, and evaluated collinearity among 34 different cyanobacteria.
Outlook: We have found some candidate proteins that bind to RNA.
Next, we need to explore these proteins experimentally by analyzing mutants and finding RNAs that bind to them.
Our data not only provide an important knowledge supplement for cyanobacteria, a unique model organism, but also provide a comprehensive resource for cyanobacteria and other photosynthetic organelles.
For details, please visit: https://sunshine.
biologie.
uni-freiburg .
de/GradSeqExplorer/ Paper link: https://bit.
ly/3njpBn4****Long press the QR code below to identify and follow***
published an online research paper entitled Analysis of a Photosynthetic Cyanobacterium Rich in Internal Membrane Systems via Gradient Profiling by Sequencing (Grad-seq) in The Plant Cell, which explored the RNA in a photosynthetic cyanobacteria -Protein complex.
Background: Cyanobacteria are a special kind of bacteria that can carry out photosynthesis similar to plants.
Similar to terrestrial plants and eukaryotic algae, they only need light, inorganic nutrients, and relatively stable environmental conditions to survive, but these factors often change and are unpredictable.
Cyanobacteria have developed corresponding mechanisms to deal with these changes.
One of these mechanisms is also a common mechanism in all life forms, which is gene regulation at the transcriptional level.
However, since it takes a certain amount of time to take effect, only this is the only mechanism.
One mechanism is usually not enough.
Therefore, post-transcriptional regulation is the same or more important mechanism.
Proteins that bind and interact with regulatory small RNAs and other RNAs are important in the process of post-transcriptional regulation, but they have been rarely studied in cyanobacteria.
Scientific question: What are the RNA-binding proteins involved in important post-transcriptional regulation processes in cyanobacteria that have not been clearly studied in other bacteria? The post-transcriptional regulation of cyanobacteria and other non-photosynthetic bacteria may be very different.
Research results: We conducted a global analysis of the main and stable RNA-protein complex in a photosynthetic cyanobacterium.
Cyanobacteria have complex intracellular membrane systems, and these membrane systems tend to bind protein complexes.
These characteristics make cyanobacteria and plants photosynthesize cells, especially chloroplasts, similar, but distinguish them from other bacteria.
We explored Synechocystis sp.
PCC 6803, the most widely studied model cyanobacterium.
We analyzed the sedimentation reaction of 65% in 3681 different proteins and 62.
2% in 4251 different transcription units by sucrose density gradient.
We calculated a support vector machine score for predicting RNA-binding proteins, and mainly analyzed proteins with unknown function that co-sedimented with RNAs.
Assuming that these proteins are more conservative in phylogenetic evolution, we searched for possible homologs in cyanobacteria, Arabidopsis, Escherichia coli, and Salmonella enterica, and evaluated collinearity among 34 different cyanobacteria.
Outlook: We have found some candidate proteins that bind to RNA.
Next, we need to explore these proteins experimentally by analyzing mutants and finding RNAs that bind to them.
Our data not only provide an important knowledge supplement for cyanobacteria, a unique model organism, but also provide a comprehensive resource for cyanobacteria and other photosynthetic organelles.
For details, please visit: https://sunshine.
biologie.
uni-freiburg .
de/GradSeqExplorer/ Paper link: https://bit.
ly/3njpBn4****Long press the QR code below to identify and follow***
