Scientists focus on the role of ddPCR technology in blood disease monitoring

At the 2019 American Society of Hematology Annual Meeting held last month, scientists from all over the world published more than 40 abstracts, highlighting the application of Bio-Rad's droplet digital PCR (ddPCR) technology

The following are some application examples that show how to use ddPCR to monitor patients with hairy cell leukemia and chronic myelogenous leukemia

ddPCR vs.

Professor Pier Luigi Zinzani and Dr.

Researchers used ddPCR technology to detect the presence of this mutation in the peripheral blood of 10 patients with hairy cell leukemia, who received cladribine treatment many years ago and completely remitted

Broccoli said: "ddPCR technology is a highly sensitive method, much more sensitive than traditional PCR technology

ddPCR vs.

In monitoring whether patients with chronic myeloid leukemia (CML) respond to tyrosine kinase inhibitors, detecting the level of the BCR-ABL1 fusion transcript is an effective method

Researchers in three different laboratories used these two methods to quantify the levels of BCR-ABL1 fusion transcripts in 37 patients

"The ddPCR kit has good reproducibility and has shown exciting results in multiple trials and reports, which shows that ddPCR technology is expected to be used routinely in the clinic to help clinicians decide when to stop treatment," Fava said

ddPCR vs.

Sanger sequencing is also a common method when detecting BCR-ABL1 mutations

Preliminary evaluation results show that the mutations identified by ddPCR are highly consistent with NGS (> 90%)