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Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler

 Last Update: 2021-02-22
 Source: Internet
 Author: User

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high pressure liquid chromatography

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Recent developments in
PCR
fluorimetry have allowed for quick quantificationof target molecules. Before the invention of fluorimetric quantitative PCR, researchers who wanted to quantify the amount of a gene in a sample did so painstakingly by limiting dilution, competitive polymerase chain reaction (PCR), or other methods, including high-performance liquid chromatography (HPLC), solid phase assays, dot blots, or immunoassay (
1
).

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