-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Affinity chromatography is a powerful technique that allows purification of proteins based on biospecific interactions using an affinity absorbent to selectively bind a target protein (
1
,
2
). Separation is achieved through a highly specific, but reversible, interaction with a complementary binding substrate (ligand) that is immobilized on a solid support (matrix). This purification approach has been developed to incorporate protein-protein interactions into the design of the affinity matrix (
3
–
3
). Affinity chromatography procedures can achieve purification levels on the order of several thousand-fold in a single separation step. The method presented here utilizes the bacteriophage PBS2 uracil-
DNA
glycosylase inhibitor (Ugi) protein as an immobilized ligand on Sepharose 4B beads for purification of mammalian mitochondrial uracil-DNA glycosylase.
