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    Home > Biochemistry News > Microbiology News > Propagation of Wild-Type and Mutant SV40

    Propagation of Wild-Type and Mutant SV40

    • Last Update: 2021-02-21
    • Source: Internet
    • Author: User
    Tags

    trypsin cell culture

    2 3 picture
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    Simian virus 40 (SV40) is routinely propagated in established kidney cell lines derived from the African green monkey (
    1
    ). Generally, one of two cell lines, BSC-1 or CV1, are used for this purpose (
    2
    ,
    3
    ). Both of these cell lines are easy to maintain in culture and can be readily frozen and recovered from frozen storage. Wild-type SV40 grows and plaques equally well in both cell lines.
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