 Home > Biochemistry News > Microbiology News > Production of Heterologous Proteins in Yeast With the Aid of the Hsp150 Carrier

Production of Heterologous Proteins in Yeast With the Aid of the Hsp150 Carrier

 Last Update: 2021-02-17
 Source: Internet
 Author: User

Tags

yeast

produced

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Proper folding, and consequently exit from the endoplasmic reticulum (ER) and secretion of heterologous exocytic proteins in yeast can be rescued by fusing the proteins to certain yeast-derived polypeptides. Biologically active mammalian glycoproteins can be produced in
Saccharomyces cerevisiae
and
Pichia pastoris
by joining them to a fragment of a natural secretory glycoprotein of
S. cerevisiae
, Hsp150Δ. The performance of the Hsp150Δ carrier in both yeasts appears to exceed that of the MFα leader, which is widely used in industrial protein production. Here we describe the use of the Hsp150Δ carrier in
P. pastoris
in both shake flask and fermentor cultivations. As a reporter protein we use the periplasmic disulfide-bonded
Escherichia coli
enzyme β-lactamase.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

bacillomycin D, CAS:76012-17-4

CAS No.: 76012-17-4

Reagent Grade

$20-22/KG FOB

Stendomycin, CAS:11006-78-3

CAS No.: 11006-78-3

Reagent Grade

$220-240/KG FOB

Human troponin (cTnI) quality control BNCC359818 BNCC

CAS No.:

$220-280/MT EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

80 20 company(94)

united states logo(227)

ups united states(221)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.