Pokeweed saponin A

Pokeweed saponin A

Esculentoside A

【Structural formula】

[Molecular formula and molecular weight] C ₂ H ₆₆ O ₁₆ ; 826.

【Source】Phytolacca plant Phytolacca acinosa Roxb.

【Properties】White powder

This product is easily soluble in water, hot methanol , ethanol , and n-butanol , but hardly soluble in lipophilic solvents such as acetone and ether

Melting point: 263～268℃

Optical rotation: [α] _D ²⁰ +56.

【Purity Check】

TLC

1.

Developing solvent chloroform - methanol - water (7:3:1) the lower layer liquid

Check the color of 10% sulfuric acid ethanol solution and check under sunlight

2.

Developing agent ethyl acetate-ethanol-water (10:2:1)

Check the color of 10% sulfuric acid ethanol solution and check under sunlight

High performance liquid chromatography

Chromatographic column Agilent C ₁₈ , 5um (4.

The mobile phase of methanol and 0.

Detector ELSD

Differential calorimetry

The starting temperature is 50°C, the end temperature is 300°C, and the heating rate is 5°C/min

【Structural identification】204

3400, 2940-2860, 1730, 1700, 1640, 1460, 1380, 1220, 1080, 1045

ESI-MS m/z: 849 [M+Na] ⁺

¹ H-NMR (C ₅ D ₅ N) δ: 1.

¹³ C-NMR (CD ₃ OD) δ: 44.
4(C-1), 71.
5(C-2), 83.
4(C-3), 43.
1(C-4), 48.
0(C-5), 18.
5(C- 6), 33.
4(C-7), 40.
6(C-8), 49.
3(C-9), 37.
5(C-10), 24.
6(C-11), 124.
2(C-12), 144.
9(C-13 ), 43.
0(C-14), 28.
8(C-15), 24.
2(C-16), 47.
0(C-17), 44.
0(C-18), 43.
3(C-19), 45.
0(C-20) , 31.
3(C-21), 35.
0(C-22), 65.
3(C-23), 14.
7(C-24), 17.
5(C-25), 17.
8(C-26), 26.
4(C-27), 181.
3(C-28), 28.
7(C-29), 178.
8(C-30), 52.
3(CH ₃ O-30), 106.
3(C-1'), 75.
1(C-2'), 76.
3(C- 3'), 78.
5(C-4'), 64.
6(C-5'), 103.
5(C-1"), 74.
6(C-2"), 78.
1(C-3"), 71.
6(C-4" ), 77.
8(C-5"), 62.
6(C-6")
.

【Storage】Dry and airtight
.

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