Northeast Agricultural University Liu Zhijing, Jiang Zhanmei, etc.: Screening of intestinal probiotic antibacterial lactic acid bacteria and research on antibacterial substances

Content Introduction Chinese Abstract: Objective: To screen out lactic acid bacteria that are suitable for the intestinal characteristics of the Chinese population, have excellent probiotics and high antibacterial activity, and explore their antibacterial active substances
.

Innovation: Lactic acid bacteria with good potential probiotics and safety have been isolated from the feces of healthy adults in China, and they have been found to have high antibacterial activity against Escherichia coli and Staphylococcus aureus
.

At the same time, it was discovered that the main antibacterial substances of Leuconostoc lactis were organic acids, and the main antibacterial substances of Lactobacillus salivarius and Lactobacillus paracasei were organic acids and bacteriocins, and the bacteriocins had broad-spectrum antibacterial activities
.

Methods: Preliminary identification of lactic acid bacteria through isolation and purification, strain morphology identification, 16SrRNA homology and phylogenetic tree analysis; Oxford cup double-layer agar diffusion method was used to screen out bacteriostatic lactic acid bacteria; evaluation by artificial gastrointestinal fluid and drug sensitivity test paper method The potential probiotics and drug resistance of lactic acid bacteria; analyze the antibacterial substances and properties of probiotic lactic acid bacteria through different enzymes, pH and heat treatment; use two-phase extraction method to purify the antibacterial substances; finally pass Tricine-SDS-PAGE Electrophoresis predicts the molecular weight of bacteriostatic substances and determines the bacteriostasis
.

Conclusion: Three strains of lactic acid bacteria with good potential probiotics and safety have been isolated and screened from the feces of healthy Chinese adults.
They have good antibacterial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923
.

The main antibacterial substances of Leuconostoc lactis are organic acids, and the main antibacterial substances of Lactobacillus salivarius and Lactobacillus paracasei are organic acids and bacteriocins with broad-spectrum antibacterial activity
.

Keyword group: intestinal lactic acid bacteria; screening; probiotics; antibacterial substances Authors: Zhijing LIU, Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU Cong XU, Ran TIAN, Wan WANG, Jiage MA, Liya GU, Fei LIU, Zhanmei JIANG, Juncai HOU.
Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances[J].
Journal of Zhejiang University Science B, 2021 , 22(7): 533-547.
http://doi.
org/10.
1631/jzus.
B2000602 The essential guide of this article: Full text download link: http:// php?doi=10.
1631/jzus.
B2000602 About this journal "Journal of Zhejiang University (English Edition) Series B: Biomedicine and Biotechnology", referred to as JZUS-B, is a comprehensive English monthly magazine for biology
.

JZUS-B since 2008 were included SCI-E, the latest impact factor of 3.
066, while being MEDLINE / PubMed, PMC, Scopus, BA, BIOSIS Previews other well-known platforms retrieval
.

The scope of articles accepted by JZUS-B covers life sciences, medicine, pharmacy, biochemistry, biotechnology and bioengineering, agronomy, ecological environment, and zoology and other related disciplines
.

Article types include Review, Article, Perspective, Viewpoint, Editorial, Correspondence and so on
.

The journal has always implemented a strict international peer review mechanism, with an average review period of 42 days and an average acceptance time of 53 days
.

Articles will be in press online immediately after acceptance, and will generally be officially published within 2 months
.

"Content-oriented, flexible format" is the characteristic of this journal
.

JZUS-B welcomes outstanding scholars at home and abroad to contribute the latest scientific research results, controversial topics, review papers, and organize the publication of hot albums
.

Journal Springer homepage: https:// Online submission address: http:// Contact Us The English version of Zhejiang University News is a number of English academic journals The collection scope covers all directions of science, engineering, agriculture and medicine.
Welcome to contribute to our journal.
You can follow our public account zdxbywb for more information
.