Nature Protocols: The Baiyang Group of the Institute of Genetics and Development publishes a method for high-throughput isolation, culture and identification of root bacteria

Original link: https:// Nature Protocols: Baiyang Group of Institute of Genetics and Development of Chinese Academy of Sciences published an article detailing the experimental procedures and analysis of high-throughput isolation, culture and identification of plant root bacteria Methods The research of plant root microbiome mainly relies on high-throughput amplicon and metagenomic sequencing technology to describe the species classification and genetic composition of the microbiome.

In situ isolation and culture of microorganisms is very important for revealing the functions of microorganisms in plant growth and health.

The combination of isolated and cultured microorganisms and sterile systems will reveal the causal relationship and interaction mechanism between root microorganisms and plant growth phenotypes, and is an important technology to promote the development of root microbiome from description to functional research.

Bai Yang's group wrote an article in Nature Protocols magazine that detailed the experimental procedures and analytical methods for high-throughput isolation, culture and identification of plant root bacteria.

This method uses high-throughput isolation and culture of bacteria from fresh plant roots, uses a gradient dilution method to increase the proportion of single bacteria, and uses double-sided label PCR amplification to identify and isolate the 16S rRNA genes of bacteria in high-throughput (Figure 1).

In order to facilitate data processing, a simple and easy-to-use biological information analysis process Culturome (https://github.
com/YongxinLiu/Culturome) and a graphical user interface web server (http://bailab.
genetics.
ac.
cn/) have been developed.
culturome/).

This method allows any research group (2-3 laboratory members, no bioinformatics expertise) to systematically cultivate plant root-related bacteria within 8-9 weeks.

Using this method, the Baiyang Group systematically established a plant root bacterial resource library (Nature, 2015), revealing the functions and mechanisms of Arabidopsis triterpenoids that selectively regulate the root microbiome (Science, 2019), as well as the coordinated root system of rice The microbiome uses soil nitrogen (Nature Biotechnology, 2019).

Figure 1.
Overview of high-throughput bacterial isolation, culture and identification methods.
This work was published online in Nature Protocols on January 13, 2021 (doi: 10.
1038/s41596-020-00444-7).

Assistant researcher Zhang Jingying and engineer Liu Yongxin of the Bai Yang group are the co-first authors.
Researcher Bai Yang of the Institute of Genetics and Development of the Chinese Academy of Sciences, and Researchers Paul Schulze-Lefert and Ruben Garrido-Oter of the Max Planck Institute of Plant Breeding in Germany are the co-corresponding authors.

Guo Xiaoxuan and Qin Yuan of Bai Yang's research group participated in this project.

The research was supported by the Strategic Leading Science and Technology Project of the Chinese Academy of Sciences, the Key Frontier Science Research Project of the Chinese Academy of Sciences, the General Program and Youth Project of the National Natural Science Foundation of China, and the Youth Innovation Promotion Association of the Chinese Academy of Sciences.

Jingying Zhang, Yong-Xin Liu, Xiaoxuan Guo, Yuan Qin, Ruben Garrido-Oter, Paul Schulze-Lefert & Yang Bai.
(2021).
High-throughput cultivation and identification of bacteria from the plant root microbiota.
Nature Protocols, doi: https://doi.
org/10.
1038/s41596-020-00444-7 Click to read the original text, jump to the article list to read and download the PDF