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Written by Dao Chang Responsibility Editor | Wangyijian microbial community is the most important part of the soil ecological environment, which has an important influence on plant growth, nutrient acquisition, and resistance to biotic and abiotic stresses [1].
Pseudomonas fluorescens has been found to play an important role in promoting plant growth and biological control of diseases [2].
At the same time, studies have shown that plants have a greater impact on the composition and population of beneficial pseudomonas colonies in the soil, but plant regulation The genetic molecular mechanism of beneficial microbial colonies in soil is still unclear.
The name of cell membrane receptor protein kinase FERONIA (FER) is derived from the Latin "goddess of spring", which plays an important role in plant growth and development, reproduction, and response to adversity stress [3], but does FERONIA participate in plant roots? The regulation of the international microbial community is unclear.
On May 10, researchers from the University of British Columbia in Canada published a research paper entitled FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species in Nature Plants, revealing that the cell membrane receptor kinase FERONIA regulates the production of ROS A new mechanism for regulating the beneficial pseudomonas flora in the rhizosphere of plants.
In this study, the researchers first identified a hormone-induced immunosuppressive mutant hsm13 through mutant screening, and found that this mutant can affect the population level of Pseudomonas fluorescens WCS365.
After sequencing technology to assist the grouping and mixed analysis of the map-based cloning data, it was determined that the hsm13 mutant was an allelic mutant of the FER gene (fer-8).
In order to confirm the function of fer-8, the 16s ribosomal RNA data of the mutant and wild-type rhizosphere microbiomes were analyzed, and it was shown that in the rhizosphere of fer-8 mutant plants, most bacteria of the Pseudomonas genus were enriched and There was no community disorder at the phylum classification level.
Through microbiome transfer experiments, it is determined that the microbial colonies enriched by fer-8 mutants can promote plant growth without causing obvious diseases to plants.
In order to study the molecular mechanism of fer-8 regulating the rhizosphere microbiome, the transcriptome of the fer-8 mutant was analyzed and found that jasmonic acid (JA) alone regulates immunity and jasmonic acid-salicylic acid (JA-SA) antagonistic regulation There is no obvious correlation between the immunity and the change of rhizosphere microbiome.
Previous studies have shown that FER regulates MAMP-induced ROS bursts and basal ROS levels in roots through small G protein (ROP2), and ROP2 is a positive regulator of NADPH oxidase on the plasma membrane [4,5].
Through genetic analysis of different gene mutants, it was found that ROP2-mediated basic level ROS regulation is a necessary condition for the growth regulation of Pseudomonas rhizosphere.
FER is the receptor of multiple RALF proteins [4,6].
Through external application and overexpression of RALF23 protein, researchers found that after the invasion of pathogens, plants may increase the population of Pseudomonas through the RALF-FER signaling pathway.
In summary, this study found a new negative regulation mechanism of Pseudomonas rhizosphere colonies, that is, FER regulates the production of basic ROS through ROP2-dependent NADPH oxidase activity, thereby inhibiting Pseudomonas colonies.
This work shows that a single regulatory factor and simple regulatory pathway can enrich the beneficial pseudomonas in the complex rhizosphere microbiota, and the research results are of great significance for guiding the practice of new agricultural breeding and microbiota engineering.
References: [1] Lugtenberg, B.
& Kamilova, F.
Plant-growth-promoting rhizobacteria.
Annu.
Rev.
Microbiol.
63, 541–556 (2009).
[2] Haas, D.
& Defago, G.
Biological control of soil-borne pathogens by fuorescent pseudomonads.
Nat.
Rev.
Microbiol.
3, 307–319 (2005) [3] Zhang, X.
, Yang, Z.
, Wu, D.
& Yu, F.
RALF–FERONIA signaling : linking plant immune response with cell growth.
Plant Commun.
1, 100084 (2020).
[4] Stegmann, M.
et al.
Te receptor kinase FER is a RALF-regulated scaffold controlling plant immune signaling.
Science 355, 287–289 (2017).
[5] Duan, Q.
, Kita, D.
, Li, C.
, Cheung, AY & Wu, HM FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.
Proc.
Natl Acad.
Sci.
USA 107, 17821–17826 (2010) [6] Xiao, Y.
et al.
Mechanisms of RALF peptide perception by a heterotypic receptor complex.
Nature 572,270–274 (2019) Link to the paper:
Pseudomonas fluorescens has been found to play an important role in promoting plant growth and biological control of diseases [2].
At the same time, studies have shown that plants have a greater impact on the composition and population of beneficial pseudomonas colonies in the soil, but plant regulation The genetic molecular mechanism of beneficial microbial colonies in soil is still unclear.
The name of cell membrane receptor protein kinase FERONIA (FER) is derived from the Latin "goddess of spring", which plays an important role in plant growth and development, reproduction, and response to adversity stress [3], but does FERONIA participate in plant roots? The regulation of the international microbial community is unclear.
On May 10, researchers from the University of British Columbia in Canada published a research paper entitled FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species in Nature Plants, revealing that the cell membrane receptor kinase FERONIA regulates the production of ROS A new mechanism for regulating the beneficial pseudomonas flora in the rhizosphere of plants.
In this study, the researchers first identified a hormone-induced immunosuppressive mutant hsm13 through mutant screening, and found that this mutant can affect the population level of Pseudomonas fluorescens WCS365.
After sequencing technology to assist the grouping and mixed analysis of the map-based cloning data, it was determined that the hsm13 mutant was an allelic mutant of the FER gene (fer-8).
In order to confirm the function of fer-8, the 16s ribosomal RNA data of the mutant and wild-type rhizosphere microbiomes were analyzed, and it was shown that in the rhizosphere of fer-8 mutant plants, most bacteria of the Pseudomonas genus were enriched and There was no community disorder at the phylum classification level.
Through microbiome transfer experiments, it is determined that the microbial colonies enriched by fer-8 mutants can promote plant growth without causing obvious diseases to plants.
In order to study the molecular mechanism of fer-8 regulating the rhizosphere microbiome, the transcriptome of the fer-8 mutant was analyzed and found that jasmonic acid (JA) alone regulates immunity and jasmonic acid-salicylic acid (JA-SA) antagonistic regulation There is no obvious correlation between the immunity and the change of rhizosphere microbiome.
Previous studies have shown that FER regulates MAMP-induced ROS bursts and basal ROS levels in roots through small G protein (ROP2), and ROP2 is a positive regulator of NADPH oxidase on the plasma membrane [4,5].
Through genetic analysis of different gene mutants, it was found that ROP2-mediated basic level ROS regulation is a necessary condition for the growth regulation of Pseudomonas rhizosphere.
FER is the receptor of multiple RALF proteins [4,6].
Through external application and overexpression of RALF23 protein, researchers found that after the invasion of pathogens, plants may increase the population of Pseudomonas through the RALF-FER signaling pathway.
In summary, this study found a new negative regulation mechanism of Pseudomonas rhizosphere colonies, that is, FER regulates the production of basic ROS through ROP2-dependent NADPH oxidase activity, thereby inhibiting Pseudomonas colonies.
This work shows that a single regulatory factor and simple regulatory pathway can enrich the beneficial pseudomonas in the complex rhizosphere microbiota, and the research results are of great significance for guiding the practice of new agricultural breeding and microbiota engineering.
References: [1] Lugtenberg, B.
& Kamilova, F.
Plant-growth-promoting rhizobacteria.
Annu.
Rev.
Microbiol.
63, 541–556 (2009).
[2] Haas, D.
& Defago, G.
Biological control of soil-borne pathogens by fuorescent pseudomonads.
Nat.
Rev.
Microbiol.
3, 307–319 (2005) [3] Zhang, X.
, Yang, Z.
, Wu, D.
& Yu, F.
RALF–FERONIA signaling : linking plant immune response with cell growth.
Plant Commun.
1, 100084 (2020).
[4] Stegmann, M.
et al.
Te receptor kinase FER is a RALF-regulated scaffold controlling plant immune signaling.
Science 355, 287–289 (2017).
[5] Duan, Q.
, Kita, D.
, Li, C.
, Cheung, AY & Wu, HM FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.
Proc.
Natl Acad.
Sci.
USA 107, 17821–17826 (2010) [6] Xiao, Y.
et al.
Mechanisms of RALF peptide perception by a heterotypic receptor complex.
Nature 572,270–274 (2019) Link to the paper:
