-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Techniques based on nucleic acid amplification have proven to be essential for the detection and epidemiological tracking of members of the genus
Cryptosporidium
. This gastrointestinal protozoan parasite cannot be routinely cultivated and it has an extremely low infectious dose, possibly below 100 oocysts. As
Cryptosporidium
is an important pathogen, particularly in immuno-compromised hosts, there is a pressing need to employ sensitive and discriminatory systems to monitor the organism. A number of fairly standard target genes have been assessed as detection targets, including 18
S
rRNA, microsatellites, and heat-shock (stress) proteins. As our knowledge of the biology of the organism increases, and as the full genome information becomes available, the choice of target may change. Genes encoding parasite-specific surface proteins (gp60, TRAP-C2, COWP) have already been examined. Much of the effort expended in molecular diagnostics of
Cryptosporidium
has been directed toward developing robust nucleic acid extraction methods. These are vital in order to recover amplifiable
DNA
from environments where small numbers of oocysts, often fewer than 100, may exist. Methodology based on adaptation of commercial kits has been developed and successfully employed to recover amplifiable DNA directly from water, food (particularly seafood), and fecal samples.
