Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction
-
Last Update: 2021-02-16
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Dictyostelium
provides a well established model system for the study of mitochondrial biology and disease. Mitochondrial dysfunction in
Dictyostelium
has been generated by knockout of nonessential nuclear genes encoding mitochondrial proteins, by knockout of targeted mitochondrial genes in a subset of the mitochondria, and by knock down of essential nuclear-encoded mitochondrial proteins. The resulting effects on mitochondrial electron transport and membrane potential can be studied by directly measuring the activities, composition, and assembly or stability of individual mitochondrial respiratory complexes and by using fluorescent probes to assay the mitochondrial membrane potential
in vivo
. Assays for these are described here. The complexities of mammalian developmental biology have obscured the phenotype-genotype relationships in mitochondrial disease and this has inhibited understanding of the underlying cytopathological mechanisms. By contrast, the
Dictyostelium
model has revealed a characteristic constellation of downstream phenotypic outcomes that, e.g., point to/show common underlying cytopathological mechanisms in mitochondrial disease. These aberrant phenotypes arise from chronic hyperactivity of the energy-sensing protein kinase AMPK and the assay of the most prominent of them is described here.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
