Major Innovations in Microbiology: Hybrid Culture of Microbes

TM: Prospect of Microbial Hybrid Culture (Summary)Trends in Microbiology (IF:11.020)(1) Since the establishment of microbiology has been limited by simple culture, and the emergence of mixed culture model has opened up another way to understand microorganisms, but also has a profound impact on microbial ecology, symbiosis, pathology and other fields; The transformation of culture depends mainly on advances in three technologies: microfluidictechnology, next-generation 3D bioprinting, single-cell metabolomics;NPJBM: Biofilm and Flora Characteristics in Colorectal Cancer TissueNPJ Biofilms and Microbiomes (IF:N/A)(1) 16S rRNA gene amplification and bio-film-based sequencing in a group of colorectal cancer (CRC) patients from Malaysia, and quantitative analysis of CRC feces and colonotoritary flora in another 11 databases The public data were analyzed at high resolution, and (2) the results confirmed that THE CRC was highly rich in the human oral flora of invasive biofilms (especially right tumors), a symbiotic symbiotic (apomorphic) and oral pathogenic bacteria (including Clostridium Difotine, digestive streptococcus, Parvimonas micra) ;(3)NPJBM: Intestinal flora characteristics associated with the metabolism of anti-colorectal cancer drugsNPJ Biofilms and Microbiomes (IF:N/A)(1) the bacteria in the gut beta-glucosalic acidase (BG) can be reactivated into the intestinal inactive Iletikon metabolic products (SN-38G) ;(2) The characteristics of the bacteria associated with metabolic type, (3) the high conversion rate of the bacterial group metabolizing type, significantly increased the risk of Iliticon-dependent adverse reactions, this type is related to certain bacteria (including the Clostridium Dorsey, the genus of Pycoccal, the uncultivated Clostridium d'orgy species) BG-related, (4) inhibiting BG in the target population can reduce the adverse reactions of Iliticon dependence; ACSID: How the intestinal flora metabolizes the anti-tumor drug amycinACS Infection Diseases ( IF: 3.600)(1) screening and analyzing the ability of the intestinal flora to inactivate the anti-tumor drug amycin; Planticola can decogenize amycin, produce metabolites 7-deoxyatoxin alcohol and 7-deoxygendoxorubinolone; (4) anaerobic conditions, amycin can also be pneumonia Krebs and Ecoli BWW5113 degradation, the degradation process requires the participation of the aphid-dependent enzyme; (5) Rplanticola to amycinicine can reduce the toxicity of the drug to the hidden wormCOP: Sequencing for pediatric infection diagnosis (overview)Current Opinion ins (IF: 2.234)(1) There is no need to prejudge the range of pathogens and there is no bias in targeted sequencing; Outbreaks and prevention of infectious diseases; (3) the time-consuming and cost-effectiveness of macrogenomics methods are decreasing, and there are good application prospects in the field of clinical infection diagnosis in pediatrics, such as detection of viral infections in the central nervous system, detection of new pathogen infections, etc.; (4) the limitations include: contamination of human DNA, difficulty of biopsy, lack of standard processes, identification of genetic material of pathogens is not equivalent to infection, and so onSR: Reliable Metabolic Syndrome Mouse Model Scientific Reports (IF: 4.259) (1) TSOD mice can spontaneously develop obesity-related syndromes; (2) more gram-positive bacteria in 24-week-old TSOD mice, less Gram-negative bacteria, and (3) And 9 genus changed; (4) total plasma short chain fatty acid (SCFA) content was significantly lower than normal mice, acetic acid content decreased, valproic acid and butyric acid content increased, and did not contain a few SCFA, such as procitine and hexaric acid; Science: The role of nitrite oxide bacteria in deep-sea carbon fixation Science( IF: 37.205) (1) using 16S rRNA gene PCR, screened 39 deep-sea oxygen reservoir samples and 2 coastal system seasonal oxygen samples of bacteria and archaea 3,463 mono-amplification genomes (SAG);(2) single-cell genomics and macroscology Genomics suggests that nitrospinae in the ocean is the most abundant and globally widely distributed nitrite oxidation bacteria (NOB) ;(3) macro proteomics and macro transcriptional almology analysis suggests that nitrite oxidation is the most important yield route of Nitrospinae gate; FM: The Earth's high sulphate environment, which has similar points to ancient Mars Frontiers in Microbiology, (1) Organisms can survive in high permeable pressure, high-salt, low-water environment, sulfates are widely deposited on Mars, it can be seen that ancient Mars is an extreme environment of salt water;) to imitate the ancient Mars experiments, through macro genome sequencing to obtain the representation of microbial structure in sediments; (3) to identify a variety of bacteria containing thick-walled bacteria, bacteria, etc., a large number of unknown bacteria and a small number of ancient bacteria, almost no DNA virus; (4) high-salt, highmineralization environment can not prevent microbial survival, on the contrary, help organic materials and biological markers retention