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    Home > Biochemistry News > Plant Extracts News > Luciferases as Reporter Genes

    Luciferases as Reporter Genes

    • Last Update: 2020-11-18
    • Source: Internet
    • Author: User
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    Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in
    Arabidopsis
    and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression. Transgenic or transiently transformed plants with a luciferase gene under the control of a promoter of interest are required. Detection of the low level of luminescence produced by the luciferase gene in
    Arabidopsis
    requires the use of low light detecting charge-coupled device (CCD) cameras or scintillation counters. This chapter contains protocols on assaying and imaging luciferase in vivo and the automation for high-resolution timecourses.
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