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Lactobacillus plantarum P-8 relieves stress and anxiety symptoms in the human body by regulating the intestinal flora

 Last Update: 2021-03-17
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Original title: The potential mechanism of Lactobacillus plantarum P-8 by regulating intestinal flora to relieve stress and anxiety symptoms in the human body

BackgroundNowadays, people's work and life pressure and other problems are common, which can easily induce the occurrence of mental illness.
You must know that mental health is one of the most important health topics at present.
According to a 2015 WHO report, nearly 300 million people worldwide suffer from anxiety disorders.
Scientific research has shown that stress conferences disturb the balance of human intestinal flora, which in turn causes mental health problems such as anxiety and depression.
As an important edible microecological agent, probiotics have been proven to reduce stress, anxiety and improve mental health problems.
In a clinical trial released in 2019, Ketuo Biopharmaceuticals has found that by taking 12 weeks of Lactobacillus plantarum P-8, it can effectively alleviate the stress and anxiety of adults.
Regarding how Lactobacillus plantarum P-8 regulates stress and anxiety through the intestinal flora, Ketuo Biotech has also conducted follow-up research to explore how Lactobacillus plantarum P-8 can relieve human stress and anxiety through the intestinal flora.
The mechanism of anxiety.
The trial designadopted a randomized, double-blind, placebo-controlled method.
79 adult volunteers aged 18-60 were selected to participate in the 12-week clinical trial.
Among them, 43 were in the probiotic group and 36 were in the placebo group.
Volunteers in the probiotic group consumed Lactobacillus plantarum P-8 (2 grams, 2x 10 ¹⁰CFU/day), and the placebo group consumed the same amount of placebo daily.
　　Stool samples (158 in total) were collected from two groups of volunteers before and after the experiment (week 0 and week 12) for DNA extraction and sequencing.
Analyze the characteristics of intestinal genome, intestinal microbial composition, related nerve compounds and metabolism.

　　Test results 　　1.
Genome characteristics of the gut microbiome A 　　total of 3,803, 851, and 1,240 high-quality, medium-quality, and partial-quality MAG (metagenomic assembled genomes) were obtained from stool samples; dRep was used for genome comparison and selected after de-redundancy There are 589 representative species-level genomes (SGBs) distributed in 13 phyla, 91 genera and 341 species.
　　At the species level, 50 SGBs are still not mapped and characterized.
These uncultured species mainly belong to three phyla, namely Firmicutes (72%), Bacteroides (11.
67%) and Proteus (4).
%).
After comparing our SGBs with the latest two databases, we found that 78 SGBs did not match the genomes in these two databases, indicating that they may be new species.

　　2.
The intestinal microbial composition 　　after the intervention of Lactobacillus plantarum P-8 was analyzed by NMDS, and it was found that the microbial structure of Lactobacillus plantarum P-8 before and after the experiment was significantly different.
Based on the Aitchison distance analysis, it was found that the Aitchison distance of the placebo group samples was significantly greater than the probiotic group samples after the test.
Compared with the 0th week, the Shannon diversity index of the placebo group decreased significantly in the 12th week, but there was no significant difference in the probiotic group.
Both the probiotic group and the placebo group showed changes in α and β diversity after the 12-week test period, but Lactobacillus plantarum P-8 had less changes in the diversity and structure of the intestinal flora.
　　In the 12th week, the contents of Bifidobacterium adolescentis, Bifidobacterium longum, and Bifidobacterium procura in the samples of the probiotic group increased significantly; while the contents of Clostridium decreased significantly.
Among them, Lactobacillus and Bifidobacterium have been found to be positively related to brain health, and play a regulatory and protective role in neurological diseases; Bifidobacterium adolescentis can play an anti-anxiety and anti-depressant effect by rebalancing the intestinal microbiota and reducing inflammatory cytokines effect.
Lactobacillus plantarum P-8 can increase the content of beneficial bacteria, which is beneficial to brain health, relieves stress and anxiety symptoms in adults, and maintains human mental health.

　　3.
The related pathways of Lactobacillus plantarum P-8 regulating intestinal nerve activity.
　　The treatment of Lactobacillus plantarum P-8 increases the diversity of SGBs, and these SGBs have potential related pathways for neural activity, and 589 SGBs are found to be related to neural activity.
Changes in pathways mainly focus on intestinal microbial metabolism related to sugar fermentation, amino acid utilization, short-chain fatty acids, and intestinal enzymes.
There are 12 phyla related to the transformation and metabolic pathways of neuroactive substances, which are mainly distributed in Firmicutes (53.
47%), Bacteroides (18.
79%) and Actinomycetes (12.
20%).
The three main pathways are carotenoid-dependent enzymes (96.
69%), conversion of acetyl-CoA to acetic acid (95.
93%) and glutamine degradation II (89.
98%).

　　4.
Lactobacillus plantarum P-8 regulates enteric neuroactive substances.
　　There are 32 and 17 different modules which are related to the synthesis and degradation of neuroactive substances, respectively.
GBMs, encoded by different SGBs, showed a significant difference between the probiotic group and the placebo group.
At the 12th week, the probiotic group found that SGBs with higher diversity were involved in vitamin K2 synthesis, short-chain fatty acid (SCFAs) and gamma-aminobutyric acid (GABA) metabolism; while the more diverse SGBs in the placebo group were involved in muscle Alcohol degradation.
In addition, compared with the placebo group, subjects in the probiotic group had more SGBs involved in the degradation of cortisol in the intestinal tract during the 12th week.
SGBs encoding histamine synthesis appeared more frequently in the intestines of subjects in the probiotic group at 12 weeks.
　　This indicates that Lactobacillus plantarum P-8 regulates the secretion of neurotransmitters or related substances through the gut-brain axis, improves the synthesis of vitamin K2, the metabolism of GABA and SCFAs, thereby reducing the occurrence of stress reactions such as anxiety and depression.
　　5.
Lactobacillus plantarum P-8 regulates intestinal nerve metabolites.
　　41 and 12 different metabolites were identified in the probiotic group and the placebo group, respectively.
There are significant differences in the predicted abundance levels of these diverse metabolites before and after the test.
At 12 weeks, the average predicted content of bile acid, arachidonic acid, creatine, threonine sphingosine, erythronic acid and sphingomyelin in the intestine of subjects in the probiotic group was significantly higher.
Among them, bile acids contribute to intestinal permeability and blood-brain barrier, and can stimulate the production of serotonin.
　　Summary 　　Lactobacillus plantarum P-8 promotes the production and secretion of some key neurotransmitters and nerve activators in the intestine by regulating the intestinal flora, and directly and indirectly regulates the intestinal-brain axis through the vagus nerve, cytokines and microbial-derived metabolites, and then Relieve adult stress and anxiety.
This also indicates that there is a potential link between the regulation of the gut microbiota induced by Lactobacillus plantarum P-8 and the relief of stress/anxiety, and supports the involvement of the gut-brain axis in alleviating stress and anxiety-related symptoms.

　　Ketuo Biotech 　　focuses on the intestinal health of the Chinese people.
Through continuous efforts, it has built the largest bacterial resource bank in Asia, and has preserved more than 20,000 strains of lactic acid bacteria and bifidobacteria.
More than 100 patents have been applied for the star strains, and more than 300 documents have been published.
　　Since 2016, Ketuo Biosciences has carried out 37 clinical trials around star strains in more than ten tertiary hospitals in China.
The clinical effects are remarkable and widely acclaimed.
　　Clinical trial number: NCT03268447 　　Article published in: Neurobiology of Stress 14 (2021) 100294

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Original title: The potential mechanism of Lactobacillus plantarum P-8 by regulating intestinal flora to relieve stress and anxiety symptoms in the human body

　　Background 　　Nowadays, people's work and life pressure and other problems are common, which can easily induce the occurrence of mental illness.
You must know that mental health is one of the most important health topics at present.
According to a 2015 WHO report, nearly 300 million people worldwide suffer from anxiety disorders.
Scientific research has shown that stress conferences disturb the balance of human intestinal flora, which in turn causes mental health problems such as anxiety and depression.
　　As an important edible microecological agent, probiotics have been proven to reduce stress, anxiety and improve mental health problems.
In a clinical trial released in 2019, Ketuo Biopharmaceuticals has found that by taking 12 weeks of Lactobacillus plantarum P-8, it can effectively alleviate the stress and anxiety of adults.
Regarding how Lactobacillus plantarum P-8 regulates stress and anxiety through the intestinal flora, Ketuo Biotech has also conducted follow-up research to explore how Lactobacillus plantarum P-8 can relieve human stress and anxiety through the intestinal flora.
The mechanism of anxiety.
　　The trial design 　　adopted a randomized, double-blind, placebo-controlled method.
79 adult volunteers aged 18-60 were selected to participate in the 12-week clinical trial.
Among them, 43 were in the probiotic group and 36 were in the placebo group.
Volunteers in the probiotic group consumed Lactobacillus plantarum P-8 (2 grams, 2x 10 ¹⁰CFU/day), and the placebo group consumed the same amount of placebo daily.
　　Stool samples (158 in total) were collected from two groups of volunteers before and after the experiment (week 0 and week 12) for DNA extraction and sequencing.
Analyze the characteristics of intestinal genome, intestinal microbial composition, related nerve compounds and metabolism.

Original title: The potential mechanism of Lactobacillus plantarum P-8 by regulating intestinal flora to relieve stress and anxiety symptoms in the human body

　　Background 　　Nowadays, people's work and life pressure and other problems are common, which can easily induce the occurrence of mental illness.
You must know that mental health is one of the most important health topics at present.
According to a 2015 WHO report, nearly 300 million people worldwide suffer from anxiety disorders.
Scientific research has shown that stress conferences disturb the balance of human intestinal flora, which in turn causes mental health problems such as anxiety and depression.
　　As an important edible microecological agent, probiotics have been proven to reduce stress, anxiety and improve mental health problems.
In a clinical trial released in 2019, Ketuo Biopharmaceuticals has found that by taking 12 weeks of Lactobacillus plantarum P-8, it can effectively alleviate the stress and anxiety of adults.
Regarding how Lactobacillus plantarum P-8 regulates stress and anxiety through the intestinal flora, Ketuo Biotech has also conducted follow-up research to explore how Lactobacillus plantarum P-8 can relieve human stress and anxiety through the intestinal flora.
The mechanism of anxiety.
　　The trial design 　　adopted a randomized, double-blind, placebo-controlled method.
79 adult volunteers aged 18-60 were selected to participate in the 12-week clinical trial.
Among them, 43 were in the probiotic group and 36 were in the placebo group.
Volunteers in the probiotic group consumed Lactobacillus plantarum P-8 (2 grams, 2x 10 ¹⁰CFU/day), and the placebo group consumed the same amount of placebo daily.
　　Stool samples (158 in total) were collected from two groups of volunteers before and after the experiment (week 0 and week 12) for DNA extraction and sequencing.
Analyze the characteristics of intestinal genome, intestinal microbial composition, related nerve compounds and metabolism.

Original title: The potential mechanism of Lactobacillus plantarum P-8 by regulating intestinal flora to relieve stress and anxiety symptoms in the human body

Original title: The potential mechanism of Lactobacillus plantarum P-8 by regulating the intestinal flora to relieve stress and anxiety symptoms in the human body Original title: The potential mechanism of action of Lactobacillus plantarum P-8 in relieving human stress and anxiety symptoms by regulating the intestinal flora Background Nowadays, people's work and life pressure and other problems are common, which can easily induce the occurrence of mental illness.
You must know that mental health is one of the most important health topics at present.
According to a 2015 WHO report, nearly 300 million people worldwide suffer from anxiety disorders.
Scientific research has shown that stress conferences disturb the balance of human intestinal flora, which in turn causes mental health problems such as anxiety and depression.
As an important edible microecological agent, probiotics have been proven to reduce stress, anxiety and improve mental health problems.
In a clinical trial released in 2019, Ketuo Biopharmaceuticals has found that by taking 12 weeks of Lactobacillus plantarum P-8, it can effectively alleviate the stress and anxiety of adults.
Regarding how Lactobacillus plantarum P-8 regulates stress and anxiety through the intestinal flora, Ketuo Biotech has also conducted follow-up research to explore how Lactobacillus plantarum P-8 can relieve human stress and anxiety through the intestinal flora.
The mechanism of anxiety.
The trial design adopted a randomized, double-blind, placebo-controlled method.
79 adult volunteers aged 18-60 were selected to participate in the 12-week clinical trial.
Among them, 43 were in the probiotic group and 36 were in the placebo group.
Volunteers in the probiotic group consumed Lactobacillus plantarum P-8 (2 grams, 2x ¹⁰CFU/day), and the placebo group consumed the same amount of placebo daily.
Stool samples (158 in total) were collected from two groups of volunteers before and after the experiment (week 0 and week 12) for DNA extraction and sequencing.
Analyze the characteristics of intestinal genome, intestinal microbial composition, related nerve compounds and metabolism.
Test results 1.
Genomic characteristics of the gut microbiomeA total of 3803, 851 and 1240 high-quality, medium-quality and partial-quality MAG (metagenomic assembled genomes) were obtained from stool samples; dRep was used for genome comparison, and a total of 589 representative species-level genomes (SGBs) were selected after de-redundancy.
They are distributed in 13 phyla, 91 genera and 341 species.
At the species level, 50 SGBs are still not mapped and characterized.
These uncultured species mainly belong to three phyla, namely Firmicutes (72%), Bacteroides (11.
67%) and Proteus (4).
%).
After comparing our SGBs with the latest two databases, we found that 78 SGBs did not match the genomes in these two databases, indicating that they may be new species.

2.
The intestinal microbial composition after the intervention of Lactobacillus plantarum P-8 was analyzed by NMDS, and it was found that the microbial structure of Lactobacillus plantarum P-8 before and after the experiment was significantly different.
Based on the Aitchison distance analysis, it was found that the Aitchison distance of the placebo group samples was significantly greater than the probiotic group samples after the test.
Compared with the 0th week, the Shannon diversity index of the placebo group decreased significantly in the 12th week, but there was no significant difference in the probiotic group.
Both the probiotic group and the placebo group showed changes in α and β diversity after the 12-week test period, but Lactobacillus plantarum P-8 had less changes in the diversity and structure of the intestinal flora.
In the 12th week, the contents of Bifidobacterium adolescentis, Bifidobacterium longum, and Bifidobacterium procura in the samples of the probiotic group increased significantly; while the contents of Clostridium decreased significantly.
Among them, Lactobacillus and Bifidobacterium have been found to be positively related to brain health, and play a regulatory and protective role in neurological diseases; Bifidobacterium adolescentis can play an anti-anxiety and anti-depressant effect by rebalancing the intestinal microbiota and reducing inflammatory cytokines effect.
Lactobacillus plantarum P-8 can increase the content of beneficial bacteria, which is beneficial to brain health, relieves stress and anxiety symptoms in adults, and maintains human mental health.
3.
The related pathways of Lactobacillus plantarum P-8 regulating intestinal nerve activityThe treatment of Lactobacillus plantarum P-8 increased the diversity of SGBs, and these SGBs have potential related pathways of neural activity, and the changes in 589 SGBs related to neural activity pathways were found, that is, mainly focused on sugar fermentation, amino acid utilization, and short chain Intestinal microbial metabolism related to fatty acids and intestinal enzymes.
There are 12 phyla related to the transformation and metabolic pathways of neuroactive substances, which are mainly distributed in Firmicutes (53.
47%), Bacteroides (18.
79%) and Actinomycetes (12.
20%).
The three main pathways are carotenoid-dependent enzymes (96.
69%), conversion of acetyl-CoA to acetic acid (95.
93%) and glutamine degradation II (89.
98%).

4.
Lactobacillus plantarum P-8 regulates enteric neuroactive substances.
There are 32 and 17 different modules which are related to the synthesis and degradation of neuroactive substances, respectively.
GBMs, encoded by different SGBs, showed a significant difference between the probiotic group and the placebo group.
At the 12th week, the probiotic group found that SGBs with higher diversity were involved in vitamin K2 synthesis, short-chain fatty acid (SCFAs) and gamma-aminobutyric acid (GABA) metabolism; while the more diverse SGBs in the placebo group were involved in muscle Alcohol degradation.
In addition, compared with the placebo group, subjects in the probiotic group had more SGBs involved in the degradation of cortisol in the intestinal tract during the 12th week.
SGBs encoding histamine synthesis appeared more frequently in the intestines of subjects in the probiotic group at 12 weeks.
This indicates that Lactobacillus plantarum P-8 regulates the secretion of neurotransmitters or related substances through the gut-brain axis, improves the synthesis of vitamin K2, the metabolism of GABA and SCFAs, thereby reducing the occurrence of stress reactions such as anxiety and depression.
5.
Lactobacillus plantarum P-8 regulates intestinal nerve metabolites.
41 and 12 different metabolites were identified in the probiotic group and the placebo group, respectively.
There are significant differences in the predicted abundance levels of these diverse metabolites before and after the test.
At 12 weeks, the average predicted content of bile acid, arachidonic acid, creatine, threonine sphingosine, erythronic acid and sphingomyelin in the intestine of subjects in the probiotic group was significantly higher.
Among them, bile acids contribute to intestinal permeability and blood-brain barrier, and can stimulate the production of serotonin.
to sum upLactobacillus plantarum P-8 promotes the production and secretion of some key neurotransmitters and nerve activators in the intestine by regulating the intestinal flora, and directly and indirectly regulates the intestinal brain axis through the vagus nerve, cytokines and microbial-derived metabolites, thereby alleviating Adult stress and anxiety.
This also indicates that there is a potential link between the regulation of the gut microbiota induced by Lactobacillus plantarum P-8 and the relief of stress/anxiety, and supports the involvement of the gut-brain axis in alleviating stress and anxiety-related symptoms.
Ketuo Biotech focuses on the intestinal health of the Chinese people.
Through continuous efforts, it has built the largest bacterial resource bank in Asia, and has preserved more than 20,000 strains of lactic acid bacteria and bifidobacteria.
More than 100 patents have been applied for the star strains, and more than 300 documents have been published.
Since 2016, Ketuo Biosciences has carried out 37 clinical trials around star strains in more than ten tertiary hospitals in China.
The clinical effects are remarkable and widely acclaimed.
Clinical trial number: NCT03268447 Article published in: Neurobiology of Stress 14 (2021) 100294

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