JEM: Revealing the effects of aging on hematopoietic stem cell function

By transplanting the elderly hematopoietic stem cells (old HSC) from mice into the bone marrow microencase of young mice (bone marrow niche, also translated as bone marrow niches), the gene expression pattern of the elderly HSC can be confirmed to return to the pattern of young hematopoietic stem cells.
, on the other hand, the function of the elderly HSC did not recover in the young bone marrow micro-environment.
-year-old HSC's apparent genome (DNA methylation) did not change significantly even in the young bone marrow micro-environment, so DNA methylation spectrum is a better indicator than older HSC gene expression patterns.
a team led by Professor Atsushi Iwama of the Department of Stem Cell and Molecular Medicine, Institute of Medical Sciences, University of Tokyo, Japan, published these world-first findings, recently published in the journal Journal of Experiment Medicine, under the title "Limited of the Limited of the Old Hematopoietic Stem cells in young bone marrow niche".
images from Journal of Experiment Medicine, 2020, doi:10.1084/jem.20192283.
Iwama said, "These results will help develop treatments for age-related blood diseases."
" focus on the changes in the elderly HSC in the bone marrow micro-environment Iwama team studied whether the elderly HSC rejuvenates in the young bone marrow micro-environment.
without pre-treatment, such as irradiation, tens of thousands of elderly hematocytes/progenition cells collected from 20-month-old mice were transplanted into eight-week-old young mice.
two months of follow-up, they collect bone marrow cells and perform fluid cell analysis.
Iwama team also transplanted HSC from 10-week-old young mice.
, the transplanted elderly HSC was isolated and RNA sequence analysis and DNA methylation analysis were performed.
found that transplanted older HSCs were less likely to produce hematocytes than younger HSCs.
also showed that even in young bone marrow micro-environments, the ability of older HSC to differentiate into erythmatic ancestral cells continued to suffer, and that the direction of differentiation was biased.
also found that transferring older HSCs to young bone marrow micro-environments did not improve their stem cell function.
more detailed analysis may find that aging studies focusing on hematopoietic stem cells, which irreversibly affect the function of older HSC, have been active in mice with bone marrow transplant models.
, the effects of aging on hematopoietic stem cells remain unclear.
, "This new study sheds light on the importance of aging on hematopoietic stem cells," said Professor Iwama.
our findings are expected to contribute to further clarifying the mechanisms of HSC aging and understanding the pathogenesies of age-related blood diseases.
" Reference: 1. Wakako Kuribayashi et al. Limited rejuvenation of aged hematopoietic stem cells in young bone marrow niche. Journal of Experiment Medicine, 2020, doi:10.1084/jem.20192283.2. Discovery of Aging mechanism for hematopoietic stem cells This article was originally published from Bio Valley, for more information please download Bio Valley APP (