JEM: Revealing the effects of aging on hematopoietic stem cell function

A team led by Professor Atsushi Iwama of the Department of Stem Cell and Molecular Medicine, Institute of Medical Sciences, University of Tokyo, Japan, published these world-first findings, recently published in the journal Journal of Experiment Medicine, under the title "Limited of the Institute of Health hepotoietic cell stems in young bone marrow niche".
photo from Journal of Experiment Medicine, 2020, doi:10.1084/jem.20192283.
Iwama said: "These results will help develop treatments for age-related blood diseases.
" focuses on the changes in the bone marrow micro-environment of the elderly HSC Iwama team studied whether the elderly HSC rejuvenates in the young bone marrow micro-environment.
without pre-treatment, such as irradiation, tens of thousands of elderly hematocytes/progenition cells collected from 20-month-old mice were transplanted into eight-week-old young mice.
two months of follow-up, they collect bone marrow cells and perform fluid cell analysis.
Iwama team also transplanted HSC, a 10-week-old young mouse.
, the transplanted elderly HSC was isolated and RNA sequence analysis and DNA methylation analysis were performed.
found that transplanted older HSCs were less likely to produce hematocytes than young HSCs.
also showed that even in young bone marrow micro-environments, the ability of older HSC to differentiate into erythmatic ancestral cells continued to suffer, and that the direction of differentiation was biased.
also found that transferring older HSCs to young bone marrow micro-environments did not improve their stem cell function.
more detailed analysis may find that aging studies focusing on hematopoietic stem cells, which irreversibly affect the function of older HSC, have been active in mice with bone marrow transplant models.
, the effects of aging on hematopoietic stem cells remain unclear.
, "This new study sheds light on the importance of aging on hematopoietic stem cells," said Professor Iwama.
our findings are expected to contribute to further clarifying the mechanisms of HSC aging and understanding the pathogenesies of age-related blood diseases.
" Reference: 1.Wakako Kuribayashi et al. Limited rejuvenation of aged hematopoietic stem cells in young bone marrow niche. Journal of Experimental Medicine, 2020, doi:10.1084/jem.20192283. 2.Discovery of aging mechanism for hematopoietic stem cells