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    Home > Biochemistry News > Microbiology News > Isolation of Mouse Thymic Dendritic Cells

    Isolation of Mouse Thymic Dendritic Cells

    • Last Update: 2021-02-16
    • Source: Internet
    • Author: User
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    The method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (1 ,2 ) and involves the following major steps:
    1. 
    Enzymatic digestion of thymic fragments with collagenase and DNase.
    2. 
    Separation of a very-low-density cell fraction (VLDF).
    3. 
    Magnetic depletion of T-lineage cells, B cells, macrophages, and granulocytes.
    4. 
    Positive selection of DCs by magnetic cell sorting (MACS).
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