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Immunofluorescence, as the name suggests, involves the visualization of proteins and structures within cells using antibodies as fluorescent probes. It has proven to be an extremely valuable technique for several reasons:
1. | Vast numbers of cells can be processed and observed in a single experiment; |
2. | Immunofluorescence facilitates the observation of individual cells and differences among them; and |
3. | Information is not only provided on the presence or absence of a protein, but also concerning its precise location within each cell. |