How to test enzyme preparation as pure preparation

Introduction: enzyme preparation is a kind of preparation commonly used in our production industry It is widely used in various fields Then, how to test the enzyme preparation as a pure preparation? What is the function of enzyme preparation? The answer to these questions is given in the safety net Enzyme preparation is a kind of substance with enzyme characteristics extracted from biology Its main function is to catalyze various chemical reactions and improve food processing methods Enzyme preparation is a kind of substance with enzyme characteristics extracted from biology Its main function is to catalyze various chemical reactions and improve food processing methods China has approved 6 kinds of papain, α - amylase preparation, refined pectinase, β - glucoamylase, etc The commonly used index to detect the quality of enzyme preparation products is generally enzyme activity, but it is not the only index The pure enzyme preparations in the enzyme preparations are: 1 Saccharase, such as amylase, cellulase, pectinase, lactase, etc.; 2 Protease, such as bromelain, figs, trypsin, etc.; 3 Lipase, such as phosphatase, sterolase, etc.; 4 Oxidoreductase, such as glucose oxidase, catalase, peroxidase, lipoxygenase, etc; 5 Isomerase, such as glucose isomerase Enzymes are mainly used in starch processing, dairy processing, fruit and vegetable processing, wine brewing, meat, egg, fish processing, bread and baked food manufacturing, food preservation and sweetener manufacturing industries How to test the enzyme preparation as a pure preparation? The main test items of enzyme preparation: enzyme activity; other routine indicators: water, particle size; health indicators: colony count, Escherichia coli, Salmonella Generally, it is a crude enzyme for industrial production, which is slightly acidic in pH, over 90% in relative humidity, ventilated and cultured at 32-35 ℃ for 36-48h, and then dried or air dried at 40 ℃ The final product of enzyme preparation can be obtained by crushing, soaking, separation, concentration, precipitation and drying The purification methods include salting out, organic solvent precipitation, isoelectric point precipitation and column chromatography Enzyme preparation is widely used What is the function of enzyme preparation? Enzyme preparation in food is a kind of food additive Its function is generally to improve food quality, prolong food preservation period, facilitate food processing and increase food nutrition Therefore, food enzyme preparation is frequently used in food If you are interested in enzyme preparation, you should pay more attention to Baibai safety net, understand food safety knowledge, help to understand enzyme preparation problem correctly, and also use food safety detector to test whether food safety meets the standard Editor in charge: he xianrob