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High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production

 Last Update: 2021-02-15
 Source: Internet
 Author: User

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A noncarotenogenic microbe
E. coli
was engineered for high production of carotenoids. To increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (
dxs, idi
and
ispDispF
) in the isoprenoid pathway were replaced with a strong bacteriophage T5 promoter (P
T5
) by using the λ-Red recombinase system in combination with the Flp/FRT site-specific recombination system for marker excision and P1 transduction for gene trait stacking. The resulting high isoprenoid flux
E. coli
can be used as a starting strain to produce various carotenoids by introducing heterologous carotenoid genes. In this study, the high isoprenoid flux
E. coli
was transformed with a plasmid carrying the β-carotene biosynthetic genes from
Pantoea stewartii
for β-carotene production
.

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