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Genomic Footprinting Using Nucleases

 Last Update: 2021-02-01
 Source: Internet
 Author: User

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reciprocal 1 2

1 2 propanediol

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Gene expression is regulated by complex mechanisms involving dynamic interactions between cis-acting elements and trans-acting factors in a highly structured chromatin environment. Investigations of protein/
DNA
interactions in vitro may not have relevance to a living cell system. To analyze events occurring at the DNA level in a living cell, Church et al. introduced the genomic footprinting procedure (
1
). The procedure was developed further (
2
) but ligation-mediated
PCR
(LM-PCR) was the breakthrough that rendered the technique accessible to many laboratories (
3
).

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