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Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry

 Last Update: 2021-02-24
 Source: Internet
 Author: User

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The ability to rapidly differentiate
Bacillus anthracis
spores from spores belonging to other
Bacillus
spp. is potentially useful for combating the intentional release of this biothreat agent. Furthermore, not all
B. anthracis
strains are fully virulent and the ability to determine the potential virulence of the endospore is also important. In this chapter, we describe a two-color flow cytometric assay capable of simultaneously identifying
B. anthracis
spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.

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