Electroporation of Dictyostelium discoideum
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Last Update: 2021-01-26
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Source: Internet
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Author: User
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Dictyostelium discoideum
has long been an intriguing model system for the study of cell type divergence during development, cell signaling, gene expression, and other cell biological problems (
1
). With the development of
DNA
-mediated transformation, research in
Dictyostelium
has entered a new era. The ability to introduce molecules into cells has allowed the analysis of promoters by transient and stable expression of reporter genes, such as luciferase and β-galactosidase (
2
,
3
). Stable transformants have also been created that overexpress normal and altered copies of important structural and regulatory proteins (
4
–
9
). Most importantly, the development of homologous gene targeting and antisense RNA inhibition of gene expression has allowed the creation of mutant cell lines lacking specific gene products (
10
,
11
). These techniques, coupled with the recently created ordered yeast artificial chromosome library, will allow large-scale mapping, fine structure mapping, and eventually sequencing of the entire genome (
12
).
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