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Determination of Oxytetracycline, Tetracycline and Chlortetracycline Residues in Livestock and Poultry Meat

 Last Update: 2021-07-05
 Source: Internet
 Author: User

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This article introduces the high performance liquid chromatography method to determine the residues of oxytetracycline, tetracycline and chlortetracycline in livestock and poultry meat (according to GB/T5009.
116-2003)
.

1.
Principle

The sample is extracted, filtered by a microporous membrane, and injected directly, separated by reversed-phase chromatography, detected by ultraviolet detector, and quantified compared with the standard.
The peak sequence is oxytetracycline , tetracycline , and chlortetracycline
.

Standard addition method quantification

Calculate as follows:

In the formula, X——the antibiotic content in the sample, mg/kg

m ₁ ——The quality of the antibiotic measured in the sample solution, ug

m ₂ ——the mass of the sample, g

Chromatographic conditions

Chromatographic column: 0DS-C18 (5um) 6.
2mm × 15cm; detection wavelength: 355nm; sensitivity: 0.
002 AUFS; column temperature: room temperature; flow rate: 1.
0 mL/min; injection volume: 10 uL; mobile phase: acetonitrile + 0.
01mol/ L sodium dihydrogen phosphate solution ( adjust pH 2.
5 with 30% nitric acid solution)=35+65, degas with ultrasonic for 10min before use
.

3.
Tips

This method is suitable for the determination of oxytetracycline, tetracycline, and chlortetracycline residues in various livestock and poultry meats
.

The detection limit was 0.

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