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Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorimetric Detection with Post-column Derivatization

 Last Update: 2021-02-17
 Source: Internet
 Author: User

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A sensitive and selective analytical method for the quantitative determination of fumonisins B
1
(FB
1
) and B
2
(FB
2
) in maize-based foods for direct human consumption is described. The method, based on high-performance liquid chromatography and fluorescence detection, presents a rapid and automated online post-column derivatization, performed with
o
-phthalaldehyde and
N
,
N
-dimethyl-2-mercaptoethylamine (Thiofluor™). A complete separation of fumonisins is achieved in less than 13 min by using a C18 column and a gradient elution. Fumonisins are extracted from the sample with a mixture of water, acetonitrile, and methanol. The filtered extract is purified by immunoaffinity column and FB
1
and FB
2
are eluted with methanol. The method has been successfully validated, and performances comply with �criteria of the Regulation EC No 401/2006.

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