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(3) Thin layer chromatography (TLC)
TLC coats a suitable stationary phase on a glass plate, plastic or aluminum substrate to form a uniform thin layer
.
After spotting and unfolding, compare the ratio shift value (Rf) with the ratio shift value (Rf) of the chromatogram obtained by the same method according to the appropriate reference substance, which is used to identify the drug, impurity inspection or content determination method
(4) Flow injection-electrochemical detection (FL-ECD)
Flow injection (FL) is to inject a certain volume of sample solution into a flowing, non-air-spaced reagent solution (or water) carrier stream.
The injected sample solution flows into the reaction coil to form an area and interact with The reagents in the carrier flow are mixed and reacted, and then enter the flow-through detector for measurement, analysis and recording
.
Agi et al.
(5) Capillary electrophoresis (capillaryelctrophoresis, CE)
CE combines the high-speed, high-resolution and flexible and efficient advantages of high-pressure electrophoresis and liquid chromatography (LC).
It can simplify sample pre-processing and automate multi-residue analysis.
However, the sample volume is too small, which limits the detection sensitivity
.
Kowalski et al.
established a CE method for the determination of CAP, TAP and FF in poultry tissues
.
The samples were extracted and deproteinized with acetonitrile, cleaned by SPE, and measured with CE
Kowalski et al.
established a CE-UVD detection method for FF in plasma
.
The sample processing is simple, does not require gradient elution and derivatization, the linear range is between 0.
Vera-Candioti et al.
established a capillary zone electrophoresis-diode array detector (CZE-DAD) analysis method for CAP in milk
.
The use of factorial and central composite design reduces the number of optimization experiments