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"Determination of 2'-fucosyllactose in food by ion chromatography" group standard released

 Last Update: 2022-03-06
 Source: Internet
 Author: User

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[ Policies and Regulations of Chemical Machinery and Equipment Network ] According to the "Administrative Measures for the Group Standard Work of the Chinese Society of Food Science and Technology" and other regulations, the group standard of T/CIFST 007-2022 "Determination of 2'-fucosyllactose in Foods by Ion Chromatography" has been approved.
It has been approved by the expert review committee and is now released
.

Chemical Machinery and Equipment Network Policies and Regulations Chemical Machinery and Equipment

This standard is drafted in accordance with the provisions of GB/T 1.
1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents"
.
This standard was drafted by: Nankai University, National Prepackaged Food Quality Inspection and Testing Center (Zhejiang), Abbott Trading (Shanghai) Co.
, Ltd.
, Beijing Sanyuan Food Co.
, Ltd.
, Danisco (China) Co.
, Ltd.
, Inner Mongolia Yili Industrial Group Co.
, Ltd.
Company, Shanghai Customs Animal, Plant and Food Inspection and Quarantine Technology Center
.

　　This standard is compiled with reference to GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
.
This standard specifies the ion chromatography method for the determination of 2'-fucosyllactose in food
.
This standard applies to the determination of 2'-fucosyllactose content in infant formula, milk powder and formula food for special medical purposes
.

　　Method principle:

　　The sample was hydrolyzed by amylotransglucosidase and β-galactosidase, filtered, separated by ion chromatography, detected by pulsed amperometric detector, and quantified by external standard method
.

　　Instruments and Equipment:

　　1.
Ion chromatography: equipped with ternary and above gradient pump, pulse amperometric detector
.
2.
Pre-column: CarboPacTM PAl Guard Column or equivalent column
.
3.
Separation column: CarboPacTMPA1 Analytical Column or equivalent column
.
4.
Analytical balance: the inductive capacity is 0.
1mg and 0.
01g
.
5.
Constant temperature water bath: 60℃±2℃
.
6.
pH meter: Accuracy is ±0.
01
.
7.
Magnetic stirrer
.
8.
Vortex mixer
.
9.
Nitrogen blower
.
10.
Ultrapure water meter
.

agitator mixer

　　Ion chromatography reference conditions:

　　1.
Chromatographic column: pre-column (4 mm×50 mm); separation column (4 mm×250 mm)
.
2.
Column temperature: 25 ℃
.
3.
Flow rate: 1mL/min
.
4.
Injection volume: 5μL
.
5.
Column pressure: 1000psi~2500psi
.
6.
Mobile phase: phase A is water, phase B is 0.
5 mol/L sodium hydroxide solution, and phase C is 0.
3 mol/L sodium acetate solution
.

　　Ampere detector reference conditions:

　　1.
Reference electrode: KCl saturated silver/silver chloride
.
2.
Working electrode: gold electrode
.
3.
Auxiliary electrode: titanium
.
4.
Waveform: carbohydrate detection four-potential waveform
.

　　Preparation of standard curve:

　　Inject the standard series of working solutions into the ion chromatograph in sequence from low to high, and measure the corresponding response value (peak area).
The average response value (peak area) of 2′-fucosyllactose in the working solutions of the two sets of standard series before and after is taken as the ordinate, and a standard curve is drawn
.
The drift of the response values (peak areas) of the upper and lower sets of standard working solutions shall not exceed 15% when the mass concentration of the standard working solution is less than or equal to 1 mg/L, and shall not exceed 10% when the mass concentration of the standard working solution is greater than 1 mg/L
.
The standard curve integration method can choose linear without forced zero-crossing or quadratic curve without forced zero-crossing
.
The correlation coefficient of the standard curve is ≥0.
999
.
The typical ion chromatogram of 2'-fucosyllactose standard solution is shown in Appendix
A.

　　Detection limit and quantification limit:

　　1.
For solid samples, the detection limit of this method for 2'-fucosyllactose is 9mg/100g, and the quantification limit is 36mg/100g
.
2.
For liquid samples, the detection limit of this method for 2'-fucosyllactose is 1 mg/100g, and the quantification limit is 4 mg/100g
.

　　Original title: "Determination of 2'-fucosyllactose in food by ion chromatography" group standard released

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