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High performance liquid chromatography ( HPLC), also known as "high pressure liquid chromatography" and "modern column chromatography" , is an important branch of chromatography.
In recent years, high performance liquid chromatography has been widely used in the separation and determination of pharmaceutical ingredients, functional ingredients of health foods, nutritional supplements, vitamins, and proteins
DTT, dithiothreitol , is a small molecule organic reducing agent .
Dithiothreitol ( DTT) is also used mainly in clinical and experimental fields.
At present, it has been reported to use dithiothreitol spectrophotometer to determine the content of cobalt ion , but DTT is very easy to be oxidized, and the sensitivity of the titration method is not high, so cobalt ion can not be used to back titrate DTT
Related link: Dithiothreitol ( DTT)