Detailed method of making anaerobic medium

Anaerobic bacteria must grow in an aerobic environment, and any medium suitable for anaerobic growth is called anaerobic mediumnouns to explainanaerobic medium: anaerobic bacteria must grow in an aerobic environment, and any medium suitable for anaerobic growth is called anaerobic mediumSpecial media: including anaerobic media and bacterial L-type mediaSuch as liver slice broth, herpes medium and other applications should be covered with liquid or solid paraffinAnaerobic bacteria with glucose liver agar need to use anaerobic culture method, resulting in more anaerobic methods, such as the cokeless non-food acid method, co-conjugation culture method and pumping methodspecialized anaerobic bacteria must grow in anaerobic conditionsThe method of anaerobic culture is to place the medium in anoxygenic environment, the other is to add reducing the reduction substance in the medium, reduce the redox potential of the medium, and close the medium surface with vaselin or paraffin, so that the medium is isolated from the outside air, so that the medium itself becomes ananoxygenic environmentThis is an anaerobic medium, such as a meat mediumthe preparation methodcommonly used mediumliquid mediumpolyvalent protein 20% trypsin 1%yeast leachate 0.5% sodium chloride 0.5%beef paste 5% systatier 0.04%chlorinated hemoglobin 0.5% solvent (beef leaching solution) dissolved p7.4solid medium plus a cent au othera LISTER (100 ml) liquid: protein 2% beef paste 5% sodium chloride 5% yeast dip 0.5% oregano heart dip liquid as solvent agar powder 0.8% adjusted PH7.4, 25-30 ml per bottle. solids: Ibid., add 20 ml or 60 degrees C to gelatin after warming and adding 15% of gelatin indicator 1 NaOH solution: 0.1N NaOH 6 ml DW 1000 ml 2 0.015% Meran solution: 0.5% Meran 3 ml DW 1000 ml 3 6% solution: glucose 6 g DW 1000 ml 1, 2, 3 solutions equivalent mixed into indicator sodium boronat (10H2O) 2.5 g sodium thiopental 2.4 g Melan 25 ml DW 650 ml