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On December 17, Beijing time, Cell published an online review article entitled "A Crisp(r) New Perspective on SARS-CoV-2 Biology" from the University of Washington.
In a blink of an eye, 2020 is about to pass, and the new crown epidemic has occurred for almost a year, but the epidemic situation is still precarious in the world.
During this year, global scientists have also made continuous efforts in various aspects to gain an in-depth understanding of the new crown virus.
.
Recently, Adam and others summarized the progress in the analysis of the biology of the new coronavirus using CRISPR technology during this year.
CRISPR-Cas9 technology has been widely used in the field of life sciences.
Single guide RNA (sgRNA,) is a single RNA molecule fused with a small piece of self-designed crRNA sequence and tracrRNA sequence scaffold.
The CRISPR-Cas9 screening system based on the sgRNA library can help us understand the function of genes and the effect of gene expression on cells.
Scientists have obtained a large amount of information on the biology of SARS-CoV-2 based on the whole genome CRISPR-Cas9 screening method.
This article describes some host factors in the process from SARS-CoV-2 contacting the cell to leaving the infected cell.
Since SARS-CoV-2 was attached to the cell surface, in addition to the previously discovered protein receptor ACE2, genes related to glycosaminoglycans (GAGs) were also screened.
GAGs are negatively charged polysaccharides that are located on the cell surface and may enhance infectivity by attracting and concentrating viruses that usually contain positively charged plaques on the cell surface.
Then SARS-CoV-2 enters the cell in two ways, one is cleaved by cathepsin L and activates the spike protein, which is endocytosed into the cell, and the other is activated by a serine protease located on the plasma membrane, TMPRSS2 The spikes bypass the endocytic pathway and fuse directly into the cell.
During the translation of SARS-CoV-2, an endoplasmic reticulum host factor TMEM41B protein was identified.
TMEM41B shapes the endoplasmic reticulum membrane into a structure that is conducive to the formation of virus replication factories.
Finally, several host factors that help SARS-CoV-2 to leave the cell were also identified, such as phosphatidylinositol kinase (PIK3C3, PICfyve) and TMEM106B to allow the virus to leave the infected cell through exocytosis.
The genes identified in these papers need further research to fully understand the SARS-CoV-2 life cycle.
Many of the genes and complexes identified in the screening are expected to become targets for anti-COVID-19 treatments.
In addition to SARS-CoV-2, some of these genes also have corresponding effects on a variety of different coronaviruses.
Therefore, this method based on whole-genome CRISPR-Cas9 screening can determine the common aspects of virus biology, so as to develop a wide range of antiviral drugs, which can help us prepare for the next viral pandemic.
I also hope that the world can get out of the new crown epidemic in the near future! Original link: https:// Plant Biotechnology Pbj Exchange Group In order to more effectively help the majority of scientific researchers to obtain relevant information, plant biotechnology Pbj specially establishes a WeChat group.
Plant Biotechnology Journal submissions and document-related questions, official account release content and official account submission questions will be concentrated in the group for answers, and at the same time, it encourages academic exchanges and collision thinking within the group.
In order to ensure a good discussion environment in the group, please add the editor WeChat first, scan the QR code to add, and then we will promptly invite you to join the group.
Tips: When adding the editor WeChat and after joining the group, please be sure to note the school or unit + name, and the PI should be noted at the end, and we will invite you to join the PI group.
In a blink of an eye, 2020 is about to pass, and the new crown epidemic has occurred for almost a year, but the epidemic situation is still precarious in the world.
During this year, global scientists have also made continuous efforts in various aspects to gain an in-depth understanding of the new crown virus.
.
Recently, Adam and others summarized the progress in the analysis of the biology of the new coronavirus using CRISPR technology during this year.
CRISPR-Cas9 technology has been widely used in the field of life sciences.
Single guide RNA (sgRNA,) is a single RNA molecule fused with a small piece of self-designed crRNA sequence and tracrRNA sequence scaffold.
The CRISPR-Cas9 screening system based on the sgRNA library can help us understand the function of genes and the effect of gene expression on cells.
Scientists have obtained a large amount of information on the biology of SARS-CoV-2 based on the whole genome CRISPR-Cas9 screening method.
This article describes some host factors in the process from SARS-CoV-2 contacting the cell to leaving the infected cell.
Since SARS-CoV-2 was attached to the cell surface, in addition to the previously discovered protein receptor ACE2, genes related to glycosaminoglycans (GAGs) were also screened.
GAGs are negatively charged polysaccharides that are located on the cell surface and may enhance infectivity by attracting and concentrating viruses that usually contain positively charged plaques on the cell surface.
Then SARS-CoV-2 enters the cell in two ways, one is cleaved by cathepsin L and activates the spike protein, which is endocytosed into the cell, and the other is activated by a serine protease located on the plasma membrane, TMPRSS2 The spikes bypass the endocytic pathway and fuse directly into the cell.
During the translation of SARS-CoV-2, an endoplasmic reticulum host factor TMEM41B protein was identified.
TMEM41B shapes the endoplasmic reticulum membrane into a structure that is conducive to the formation of virus replication factories.
Finally, several host factors that help SARS-CoV-2 to leave the cell were also identified, such as phosphatidylinositol kinase (PIK3C3, PICfyve) and TMEM106B to allow the virus to leave the infected cell through exocytosis.
The genes identified in these papers need further research to fully understand the SARS-CoV-2 life cycle.
Many of the genes and complexes identified in the screening are expected to become targets for anti-COVID-19 treatments.
In addition to SARS-CoV-2, some of these genes also have corresponding effects on a variety of different coronaviruses.
Therefore, this method based on whole-genome CRISPR-Cas9 screening can determine the common aspects of virus biology, so as to develop a wide range of antiviral drugs, which can help us prepare for the next viral pandemic.
I also hope that the world can get out of the new crown epidemic in the near future! Original link: https:// Plant Biotechnology Pbj Exchange Group In order to more effectively help the majority of scientific researchers to obtain relevant information, plant biotechnology Pbj specially establishes a WeChat group.
Plant Biotechnology Journal submissions and document-related questions, official account release content and official account submission questions will be concentrated in the group for answers, and at the same time, it encourages academic exchanges and collision thinking within the group.
In order to ensure a good discussion environment in the group, please add the editor WeChat first, scan the QR code to add, and then we will promptly invite you to join the group.
Tips: When adding the editor WeChat and after joining the group, please be sure to note the school or unit + name, and the PI should be noted at the end, and we will invite you to join the PI group.
