Black crankmold: intracellular metabolomics sample preparation method based on liquid-based concomcity

Dynamic metabolomics is an important method to reveal the mechanism of intracellular metabolism regulation and discover metabolic bottlenecks, and the metabolomic sample preparation method will directly affect the authenticity of metabolomic data, but there is no reliable standardized sample preparation method in many important industrial strainsRecently, the research team and researcher Zheng Ping of the Center for Systembiology led by Sun Yibin, a researcher at the Tianjin Institute of Industrial Biotechnology of the Chinese Academy of Sciences, led the research team of systems and synthetic biotechnology, and optimized the preparation method of intracellular metabolic group samples based on liquid-based conjunction: using rapid filtration and liquid filtration, in view of the important industrial strain of helicomycinThe quenching method combined with nitrogen freezing can greatly reduce the recovery rate of intracellular metabolites guaranteed by cell damage, and the extraction of intracellular metabolites by using -20 oC acidic 50% acetylene combined with 100 oC 75% ethanol can obtain complete non-preferred intracellular metabolitesusing the metabolomics sample preparation method, the research system analyzed the dynamic intracellular metabolic spectrum changes in the fermentation process of black cyclic citric acid, and found that the sufficient supply of precursors such as acetone acid, oxayl acetic acid and malic acid was the key to the rapid accumulation of citric acidThe intracellular metabolomics sample preparation method established in this study provides a standardized technical process for the study of the nemesis metabolomica group and lays a solid foundation for the research of the systematic metabolic engineering of helutomycinthe study was supported by the National Natural Science Foundation of China (31700085 and 31370113) and the Tianjin Science and Technology Program (15PTCYSY00020), and the results have been published in the journal Biotechnology JournalZheng Xiaomei, an assistant researcher at Tianjin Institute of Engineering and Technology, is the first author of the paperSource: Tianjin Institute of Industrial Biotechnology