Arbitrarily Primed PCR Methods for Studying Bacterial Diseases
-
Last Update: 2021-02-15
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
The most common application of the polymerase chain reaction (
PCR
) IS to exponenttally amplify a specific known and predictable sequence from a complex mixture of nucleic acids. This chapter describes a technique that, in contrast, uses PCR with arbitrary primers, to generate a fingerprint of PCR products from complex mixtures of nucleic acids and identify sequence polymorphisms and other differences that distinguish them. This robust and reproducible technique can amplify discrete sets of
DNA
fragments. When genomic DNAs from different individuals are compared, these differences represent point mutations and variously sized deletions and insertions When different sources of RNA are examined from an isogenic source, differences in the particular set of DNA fragments amplified represent differentially expressed genes.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
