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Speaking of reverse transcriptase, SuperScript is a classic product that must be mentioned
According to Life Technologies, SuperScript® IV reverse transcriptase significantly improves resistance to a variety of inhibitors and can perform reliable and specific cDNA synthesis for a variety of sample types
Everyone knows that common inhibitory components in RNA samples can affect the cDNA synthesis process and give false negative RT-qPCR results
Compared with SuperScript® III reverse transcriptase and other reverse transcriptases, SuperScript® IV reverse transcriptase has significant resistance to polluting inhibitors
In addition, we sometimes inevitably encounter some difficult RNA samples, such as RNA from plant samples or degraded RNA
Of course, the secondary stem-loop structure of RNA and the GC-rich RNA template will also affect cDNA synthesis
Compared with SuperScript® III reverse transcriptase, in addition to the optimized resistance to inhibitors and buffers, a more obvious advantage is that the reaction time is greatly shortened
Today, SuperScript® IV reverse transcriptase is officially on the market and can be provided as a stand-alone enzyme or as a first-strand synthesis kit
However, the good news is that you can now apply for free 10 reaction samples, which can cost more than 1,000 yuan for a single purchase
Free trial package of SuperScript IV reverse transcriptase
